Figure 2. Western blot analysis using the Diagenode antibody directed against SOX4
A. H1299 lung carcinoma cells were transfected with HA-tagged SOX4 (lane 1) or with a HA-tagged truncated SOX4 (395X mutant, lane 2). Whole cell extracts (60 - 100 μg) were analysed by Western blot using the Diagenode antibody against SOX4 (Cat. No. CS-129-100) diluted 1:4,000 in TBS-Tween containing 2% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
B. Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with the Diagenode antibody against SOX4 as described above. The H522 cells are known to overexpress SOX4. Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.