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SOX4 polyclonal antibody - Pioneer

カタログ番号
フォーマット
価格
C15310129
(CS-129-100)
100 µl
$220.00
  Bulk order
ブローカー情報



Alternative names: EVI16

Polyclonal antibody raised in rabbit against human SOX4 (SRY (sex determining region Y)-box 4), using two KLH-conjugated synthetic peptides containing a sequence from the N-terminus and from the C-terminus of the protein, respectively.

LotA574-004
ConcentrationNot determined
Species reactivityHuman, mouse: positive. Other species: not tested.
TypePolyclonal
PurityWhole antiserum
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferWhole antiserum from rabbit containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:1,000 Fig 1
Western Blotting 1:4,000 Fig 2, 3
IP 6 µl/IP Fig 4

  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human SOX4 (Cat. No. C15310129). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:18,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against SOX4
    A.
    H1299 lung carcinoma cells were transfected with HA-tagged SOX4 (lane 1) or with a HA-tagged truncated SOX4 (395X mutant, lane 2). Whole cell extracts (60 - 100 µg) were analysed by Western blot using the Diagenode antibody against SOX4 (Cat. No. C15310129) diluted 1:4,000 in TBS-Tween containing 2% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
    B. Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with the Diagenode antibody against SOX4 as described above. The H522 cells are known to overexpress SOX4. Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.

    Western blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against SOX4
    Western blot was performed on Protein extracts (~40 µg) from mouse B16-OVA (A), CT-2A (B), GL261 (C) or B16A (D) cells using the Diagenode antibody against SOX4 (Cat. No. C15310129). The cells were transfected with a SOX4 specific crRNA (lanes 2) or with a negative control crRNA (lanes 1). The antibody was diluted 1:4,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right. The lower panel shows the WB results with a GAPDH antibody used as loading control. Western blot performed by Anže Godicelj, Dana Farber Cancer Institute, Boston, USA.

    IP

    Figure 4. IP using the Diagenode antibody directed against SOX4
    IP was performed overnight on protein extracts (600 µg) from CT-2A (A) and GL261 (B) cells using 6 µl of the Diagenode antibody against SOX4 (Cat. No. C15310129). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 2 shows the result of the IP; the input is shown in lane 1.

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
  •  資料
    Datasheet SOX4 CS-129-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SOX4 polyclonal antibody - Pioneer (Diagenode Cat# C15310129 Lot# A574-004 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    SOX4 can redirect TGF-β-mediated SMAD3-transcriptional output in a context-dependent manner to promote tumorigenesis.
    Vervoort SJ, Lourenço AR, Tufegdzic Vidakovic A, Mocholi E, Sandoval JL, Rueda OM, Frederiks C, Pals C, Peeters JGC, Caldas C, Bruna A, Coffer PJ
    Expression of the transcription factor SOX4 is often elevated in human cancers, where it generally correlates with tumor-progression and poor-disease outcome. Reduction of SOX4 expression results in both diminished tumor-incidence and metastasis. In breast cancer, TGF-β-mediated induction of SOX4 has been shown...

    SOX4 inhibits oligodendrocyte differentiation of embryonic neural stem cells in vitro by inducing Hes5 expression
    Braccioli Luca, Vervoort Stephin J., Puma Gianmarco, Nijboer Cora H., Coffer Paul J.
    SOX4 has been shown to promote neuronal differentiation both in the adult and embryonic neural progenitors. Ectopic SOX4 expression has also been shown to inhibit oligodendrocyte differentiation in mice, however the underlying molecular mechanisms remain poorly understood. Here we demonstrate that SOX4 regulates tra...

    TRPM7 controls mesenchymal features of breast cancer cells by tensional regulation of SOX4.
    Kuipers AJ, Middelbeek J, Vrenken K, Pérez-González C, Poelmans G, Klarenbeek J, Jalink K, Trepat X, van Leeuwen FN
    Mechanically induced signaling pathways are important drivers of tumor progression. However, if and how mechanical signals affect metastasis or therapy response remains poorly understood. We previously found that the channel-kinase TRPM7, a regulator of cellular tension implicated in mechano-sensory processes, is re...

    Cancer cell specific inhibition of Wnt/β-catenin signaling by forced intracellular acidification.
    Melnik S, Dvornikov D, Müller-Decker K, Depner S, Stannek P, Meister M, Warth A, Thomas M, Muley T, Risch A, Plass C, Klingmüller U, Niehrs C, Glinka A
    Use of the diabetes type II drug Metformin is associated with a moderately lowered risk of cancer incidence in numerous tumor entities. Studying the molecular changes associated with the tumor-suppressive action of Metformin we found that the oncogene , which is upregulated in solid tumors and associated with poor p...

    Sox4 Expression Confers Bladder Cancer Stem Cell Properties and Predicts for Poor Patient Outcome
    Shen H et al.
    Genetic and epigenetic alterations have been identified as to contribute directly or indirectly to the generation of transitional cell carcinoma of the urinary bladder (TCC-UB). We have previously found that amplification of chromosome 6p22 is significantly associated with the muscle-invasive rather than superficial...

    Novel transcriptional targets of the SRY-HMG box transcription factor SOX4 link its expression to the development of small cell lung cancer.
    Castillo SD, Matheu A, Mariani N, Carretero J, Lopez-Rios F, Lovell-Badge R, Sanchez-Cespedes M
    The HMG box transcription factor SOX4 involved in neuronal development is amplified and overexpressed in a subset of lung cancers, suggesting that it may be a driver oncogene. In this study, we sought to develop this hypothesis including by defining targets of SOX4 that may mediate its involvement in lung cancer. Ab...

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