Diagenode

RbAp48 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200206
100 µg
$350.00
  Bulk order

Alterantive names: RBBP4, CAF-1 subunit C, CAF-I P48, NURF55, Lin-53.

Monoclonal antibody raised in mouse against retinoblastoma binding protein 4, using a KLH-conjugated synthetic peptide.

Lot40186
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein G purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:500 Fig 3
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:100 - 1:500 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune mouse IgG B. 5 μg of RbAp48 antibody (C15200206) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.

    Immunoprecipitation

    Figure 2. Immunoprecipitation
    RbAp48 antibody immunoprecipitates RbAp48 protein in IP experiments. IP Sample: 1,000 μg HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2.5 μg of preimmune mouse IgG C. Immunoprecipitation of RbAp48 protein with 2.5 μg of RbAp48 antibody (C15200206) The immunoprecipitated RbAp48 protein was detected by western blot with the RbAp48 antibody diluted 1:500.

    Immunofluorescence

    Figure 3. Immunofluorescent analysis
    Immunofluorescence analysis of HeLa cells, using RbAp48 (Cat. No. C15200206) antibody at a 1:100 dilution.

    Western Blot

    Figure 4. Western blot
    Detection of RbAp48 in HeLa nuclear extract.

  • Applications
    Epigenetics DNA Methylation
    Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data betwee... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet RbAp48 C15200206 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: RbAp48 monoclonal antibody - Classic (Diagenode Cat# C15200206 Lot# 40186). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    PKC-mediated phosphorylation of BCL11B at Serine 2 negatively regulates its interaction with NuRD complexes during CD4+ T cell activation
    Dubuissez M et al.
    The transcription factor BCL11B/CTIP2 is a major regulatory protein implicated in various aspects of development, function and survival of T cells. MAPK-mediated phosphorylation and SUMOylation modulate BCL11B transcriptional activity, switching it from a repressor in naïve murine thymocytes to a transcriptiona...

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