PPARG polyclonal antibody - Pioneer

Catalog Number
100 µl
  Bulk order

Alternative names: PPAR-gamma, NR1C3

Polyclonal antibody raised in rabbit against human PPARG (peroxisome proliferator-activated receptor gamma), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:500 Fig 1
Western Blotting 1:500 Fig 2
  • Validation Data


    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PPARG (Cat. No. C15310133). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:27,600.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against PPARG
    293T cells were analysed by Western blot using the Diagenode antibody against PPARG (Cat. No. C15310133) diluted 1:500. Figure 2 shows the result of 293T cells transfected with pNTAP-PPARG (lane 2) or with an empty vector (lane 1). The position of the protein of interest is indicated on the right (expected size: 58 kDa); the marker (in kDa) is shown on the left.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    C15310133 PPARG polyclonal DATASHEET
    Datasheet description
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PPARG polyclonal antibody - Pioneer (Diagenode Cat# C15310133 Lot# A576-001). Click here to copy to clipboard.

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