Request a quote for a bulk order for PPARG polyclonal antibody - Pioneer. Please fill out the form here below. Your local sales account manager will get in touch with you
shortly and send you a quotation based on your requirements.
Polyclonal antibody raised in rabbit against human PPARG (peroxisome proliferator-activated receptor gamma), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PPARG (Cat. No. C15310133). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:27,600.
Figure 2. Western blot analysis using the Diagenode antibody directed against PPARG 293T cells were analysed by Western blot using the Diagenode antibody against PPARG (Cat. No. C15310133) diluted 1:500. Figure 2 shows the result of 293T cells transfected with pNTAP-PPARG (lane 2) or with an empty vector (lane 1). The position of the protein of interest is indicated on the right (expected size: 58 kDa); the marker (in kDa) is shown on the left.
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
Learn more about: Load... Read more