Diagenode

PML polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410108
(pAb-108-050)
50 µg/360 µl
$195.00
  Bulk order

Alternative names: MYL, PP8675, RNF71, TRIM19

Polyclonal antibody raised in rabbit against human PML (Promyelocytic leukemia), using 3 different KLH-conjugated synthetic peptides.

LotA136-0011
Concentration0.14 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:200 Fig 1
Western Blotting 1:750 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against PML
    Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 μg) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet PML pAb-108-05 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PML polyclonal antibody - Pioneer (Diagenode Cat# C15410108 Lot# A136-0011 ). Click here to copy to clipboard.

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