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Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against PML Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 μg) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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