The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from 3 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design.
The system performance was very good. High molecular weight DNA of different plants (Arabidopsis Alm, Hops polaris and wine Villard Blanc) was isolated and diluted to 50ng/µl or 70ng/µl. We aimed for fragment length 30kb at the volume 150µl.Christa Lanz and Julia Hildebrandt, Max Planck Institute for Developmental Biology, Tübingen, Germany
We achieved good results and we will continue with bead purification and library preps for PacBio sequencing. I like the user friendly interface of the Megaruptor® 2. Just two parameters had to be specified: volume of each sample and desired mean fragment size. The processed DNA has a narrow size distribution at around 30kb.