Diagenode

Megaruptor® 2

Megaruptor 2
Tight fragment size distribution
Hydropores eliminate clogging
High quality libraires
All-in-one desktop device
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Catalog Number
Format
B06010002
1 unit

The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from 3 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. 

TESTIMONIAL

The system performance was very good. High molecular weight DNA of different plants (Arabidopsis Alm, Hops polaris and wine Villard Blanc) was isolated and diluted to 50ng/µl or 70ng/µl. We aimed for fragment length 30kb at the volume 150µl.
We achieved good results and we will continue with bead purification and library preps for PacBio sequencing.  I like the user friendly interface of the Megaruptor® 2. Just two parameters had to be specified: volume of each sample and desired mean fragment size. The processed DNA has a narrow size distribution at around 30kb.

Christa Lanz and Julia Hildebrandt, Max Planck Institute for Developmental Biology, Tübingen, Germany
  • Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2
    Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. A: DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. B: Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).
    * indicates unsheared DNA
  • Applications
    Next Generation Sequencing
    DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 kb to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA libr... Read more
    DNA/RNA shearing
    In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critic... Read more
  • Documents
    Megaruptor® 2 Quick guide MANUAL
    We have designed the Megaruptor® 2 using our highest level of knowledge in shearin...
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    Megaruptor® 2 Manual MANUAL
    The Megaruptor® 2 was designed to provide researchers with a simple, automated, and r...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Megaruptor® 2 (Diagenode Cat# B06010002). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    DNA fragmentation and quality control analysis
    Wassim Lakhal, Irina Panteleeva, Sharon Squazzo, Rini Saxena, Jerome Kroonen, Steve Siembieda, Markus Tilmes & Jonathan Hagopian
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, namely, precise DNA fragmentation, size-range analysis, and smear quantification. The Diagenode One, Bioruptor®, Megaruptor®, and Fragment Analyzer™ by Advanced Analytical ensure that these first cri...

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