Diagenode

Megaruptor® 2

Megaruptor 2
Tight fragment size distribution
Hydropores eliminate clogging
High quality libraires
All-in-one desktop device
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カタログ番号
フォーマット
B06010002
1 unit

Megaruptor®2は、3kb75kbDNA断片化を行い、理想のDNA断片化を実現するために設計されました。 せん断性能は、DNAサンプルの供給源、濃度、温度、または塩分とは無関係です。追加ユーザー入力不要、分かりやすいインターフェースで相互汚染の心配なしに2つのサンプルを連続して処理することが可能です。 パラメータを設定するだけで、あとは自動システムにお任せください。また新設計により、目詰まりの心配もありません。

TESTIMONIAL

Established in March 15th 2000, DNA Link, Inc. has developed as one of the leading full genomic service provider with its extensive 17 years of experience. DNA Link has been involved in numerous genomic researches including many national projects, and contributed to the expansion of the genomic industry.

On one hand, DNA Link was the very first company to introduce the RS II system from Pacific Bioscience in Asia, and has been approved as one of the eight global certified service providers for PacBio. Since 2012, DNA Link has been providing quality results that are controlled under strict criteria and successfully assisted numerous research projects.

PacBio platforms’ strongest upside is that it generates the longest reads among all sequencing platforms, and constructing long-read libraries using high molecular weight DNA is the most crucial part of utilizing the platforms. Within the PacBio library preparation process, shearing the DNA molecules accordingly to the library size is the initial step for a successful library construction. Sufficient library yield can be obtained only if the DNA molecules are sheared within the targeted size.

DNA Link has been using the Megaruptor instrument from Diagenode for this shearing process, as it is easy to use and has less contamination risk for using disposable hydropores, which contributes to the overall efficiency of the laboratory logistics.

Read our new Nature Methods publication about the Megaruptor 2 and best NGS library preparation practices

Hyeyoon, Jang - Lab Manager - Seoul, Korea
  • Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2
    Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. A: DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. B: Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).
    * indicates unsheared DNA
  • お客様の声

    The system performance was very good. High molecular weight DNA of different plants (Arabidopsis Alm, Hops polaris and wine Villard Blanc) was isolated and diluted to 50ng/µl or 70ng/µl. We aimed for fragment length 30kb at the volume 150µl.
    We achieved good results and we will continue with bead purification and library preps for PacBio sequencing.  I like the user friendly interface of the Megaruptor® 2. Just two parameters had to be specified: volume of each sample and desired mean fragment size. The processed DNA has a narrow size distribution at around 30kb.

    Christa Lanz and Julia Hildebrandt, Max Planck Institute for Developmental Biology, Tübingen, Germany
  • アプリケーション
    次世代シーケンシング
    DNAせん断、ライブラリー作成、自動化:NGSのワンストップショップ 1. せん断装置を選択してください:150 bp〜75 kbの範囲でDNAをせん断します。 5μlまでせん断:150 bp〜2 kbNGS DNAライブラリー調製およびFFPE核酸抽出に最適で、 2 kb〜75 kbの範囲をせんできます。メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイス... Read more
    DNA/RNA断片
    近年の次世代シーケンシング(NGS)の進歩はゲノミクスや生物学に革命をもたらしました。 この成長により、最適なサンプル調製、ゲノムライブラリー構築には欠かせない高度技術の要求、必要性がさらに高まっています。 最適なライブラリー調製に最も重要な要因の一つは、シーケンシングされるDNAの品質です。 まずは、DNAを効果的かつ一貫してシーケンシングプラットフォームに応じた適切な断片サイズに切断し、具体的で信頼できるNGS結果を実現する必要があります。 Picoruptor and Megar... Read more
  • 資料
    Megaruptor® 2 Quick guide MANUAL
    We have designed the Megaruptor® 2 using our highest level of knowledge in shearin...
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    Megaruptor® 2 Manual MANUAL
    The Megaruptor® 2 was designed to provide researchers with a simple, automated, and r...
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    Megaruptor 2 Flyer FLYER
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    Application of the Megaruptor® for shearing of ultra-long DNA fragments in the context of Long Read Sequencing using 3rd Gen Sequencing Technologies APPLICATION NOTE
    The Megaruptor® provides a simple, robust, and straight forward method for reproducible DNA s...
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    Megaruptor genomic DNA shearing for large insert sequencing library preparation APPLICATION NOTE
    Wageningen University has a strong background in plant genome sequencing including contributions ...
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    Bioruptor® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
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    Optimal long DNA fragment generation prior to SMRTbell® library preparation APPLICATION NOTE
    Several methods exist today for shearing genomic DNA, but few are reliable for generating DNA fra...
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  • 出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Megaruptor® 2 (Diagenode Cat# B06010002). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Single-Molecule Sequencing Reveals the Chromosome-Scale Genomic Architecture of the Nematode Model Organism Pristionchus pacificus.
    Rödelsperger C. et al.
    The nematode Pristionchus pacificus is an established model for integrative evolutionary biology and comparative studies with Caenorhabditis elegans. While an existing genome draft facilitated the identification of several genes controlling various developmental processes, its high degree of fragmentation complicate...

    De novo PacBio long-read and phased avian genome assemblies correct and add to reference genes generated with intermediate and short reads
    Korlach J. et al.
    Reference-quality genomes are expected to provide a resource for studying gene structure, function, and evolution. However, often genes of interest are not completely or accurately assembled, leading to unknown errors in analyses or additional cloning efforts for the correct sequences. A promising solution is long-r...

    DNA fragmentation and quality control analysis
    Wassim Lakhal, Irina Panteleeva, Sharon Squazzo, Rini Saxena, Jerome Kroonen, Steve Siembieda, Markus Tilmes & Jonathan Hagopian
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, namely, precise DNA fragmentation, size-range analysis, and smear quantification. The Diagenode One, Bioruptor®, Megaruptor®, and Fragment Analyzer™ by Advanced Analytical ensure that these first cri...

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  • The 12th Annual Meeting of the Japanese Society for Epigenetics
    Jun 24-Jun 25, 2018
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