Diagenode

Megaruptor® 2

Megaruptor 2
Tight fragment size distribution
Hydropores eliminate clogging
High quality libraires
All-in-one desktop device
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Catalog Number
Format
B06010002
1 unit

The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from 3 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design.

PROMOTION Megaruptor 2 - get one year's consumables for free! (valid until: September 30, 2017)

TESTIMONIAL

Established in March 15th 2000, DNA Link, Inc. has developed as one of the leading full genomic service provider with its extensive 17 years of experience. DNA Link has been involved in numerous genomic researches including many national projects, and contributed to the expansion of the genomic industry.

On one hand, DNA Link was the very first company to introduce the RS II system from Pacific Bioscience in Asia, and has been approved as one of the eight global certified service providers for PacBio. Since 2012, DNA Link has been providing quality results that are controlled under strict criteria and successfully assisted numerous research projects.

PacBio platforms’ strongest upside is that it generates the longest reads among all sequencing platforms, and constructing long-read libraries using high molecular weight DNA is the most crucial part of utilizing the platforms. Within the PacBio library preparation process, shearing the DNA molecules accordingly to the library size is the initial step for a successful library construction. Sufficient library yield can be obtained only if the DNA molecules are sheared within the targeted size.

DNA Link has been using the Megaruptor instrument from Diagenode for this shearing process, as it is easy to use and has less contamination risk for using disposable hydropores, which contributes to the overall efficiency of the laboratory logistics.

Read our new Nature Methods publication about the Megaruptor 2 and best NGS library preparation practices

Hyeyoon, Jang - Lab Manager - Seoul, Korea
  • Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2
    Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. A: DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. B: Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).
    * indicates unsheared DNA
  • Testimonials

    The system performance was very good. High molecular weight DNA of different plants (Arabidopsis Alm, Hops polaris and wine Villard Blanc) was isolated and diluted to 50ng/µl or 70ng/µl. We aimed for fragment length 30kb at the volume 150µl.
    We achieved good results and we will continue with bead purification and library preps for PacBio sequencing.  I like the user friendly interface of the Megaruptor® 2. Just two parameters had to be specified: volume of each sample and desired mean fragment size. The processed DNA has a narrow size distribution at around 30kb.

    Christa Lanz and Julia Hildebrandt, Max Planck Institute for Developmental Biology, Tübingen, Germany
  • Applications
    次世代シーケンシング
    DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 bp to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA libr... Read more
    DNA/RNA断片
    In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critic... Read more
  • Documents
    Megaruptor® 2 Quick guide MANUAL
    We have designed the Megaruptor® 2 using our highest level of knowledge in shearin...
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    Megaruptor® 2 Manual MANUAL
    The Megaruptor® 2 was designed to provide researchers with a simple, automated, and r...
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    Megaruptor 2 Flyer FLYER
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    Application of the Megaruptor® for shearing of ultra-long DNA fragments in the context of Long Read Sequencing using 3rd Gen Sequencing Technologies APPLICATION NOTE
    The Megaruptor® provides a simple, robust, and straight forward method for reproducible DNA s...
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    Megaruptor genomic DNA shearing for large insert sequencing library preparation APPLICATION NOTE
    Wageningen University has a strong background in plant genome sequencing including contributions ...
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    Bioruptor® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Megaruptor® 2 (Diagenode Cat# B06010002). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Comparative Analysis of Ralstonia solanacearum Methylomes
    Erill I. et al.
    Ralstonia solanacearum is an important soil-borne plant pathogen with broad geographical distribution and the ability to cause wilt disease in many agriculturally important crops. Genome sequencing of multiple R. solanacearum strains has identified both unique and shared genetic traits influencing their evolution an...

    DNA fragmentation and quality control analysis
    Wassim Lakhal, Irina Panteleeva, Sharon Squazzo, Rini Saxena, Jerome Kroonen, Steve Siembieda, Markus Tilmes & Jonathan Hagopian
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, namely, precise DNA fragmentation, size-range analysis, and smear quantification. The Diagenode One, Bioruptor®, Megaruptor®, and Fragment Analyzer™ by Advanced Analytical ensure that these first cri...

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