- Sterile
- Natural
- Autoclavable
- Freezable
Storage
Store at room temperature
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures
Storage
Store at room temperature
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures
| Megaruptor® DNA Shearing System User Manual v2 MANUAL The Megaruptor® was designed to provide researchers with a simple, automated, and repro... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Hydro Tubes (Diagenode Cat# C30010018). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Hydro Tubes', 'meta_title' => 'Hydro Tubes', 'product' => array( 'Product' => array( 'id' => '2647', 'antibody_id' => null, 'name' => 'Hydro Tubes', 'description' => '<p>Megaruptor®専用の<span style="font-weight: 400;">Hydro Tubes</span>（0.5ml）は、Megaruptor®（B06010001）との使用が検証されており、DNA断片化関連実験にはMegaruptor®と併用が強く推奨されています。</p>', 'label1' => '特徴', 'info1' => '<ul> <li>Sterile</li> <li>Natural</li> <li>Autoclavable</li> <li>Freezable</li> </ul> <p></p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '50 pc', 'catalog_number' => 'C30010018', 'old_catalog_number' => '', 'sf_code' => 'C30010018-1860', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '65', 'price_USD' => '65', 'price_GBP' => '60', 'price_JPY' => '10180', 'price_CNY' => '', 'price_AUD' => '162', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydro-tubes-50-pc', 'meta_title' => 'Hydro Tubes', 'meta_keywords' => '', 'meta_description' => 'Hydro Tubes', 'modified' => '2020-04-07 05:19:42', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'Hydro Tubes' $meta_title = 'Hydro Tubes' $product = array( 'Product' => array( 'id' => '2647', 'antibody_id' => null, 'name' => 'Hydro Tubes', 'description' => '<p>Megaruptor®専用の<span style="font-weight: 400;">Hydro Tubes</span>（0.5ml）は、Megaruptor®（B06010001）との使用が検証されており、DNA断片化関連実験にはMegaruptor®と併用が強く推奨されています。</p>', 'label1' => '特徴', 'info1' => '<ul> <li>Sterile</li> <li>Natural</li> <li>Autoclavable</li> <li>Freezable</li> </ul> <p></p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '50 pc', 'catalog_number' => 'C30010018', 'old_catalog_number' => '', 'sf_code' => 'C30010018-1860', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '65', 'price_USD' => '65', 'price_GBP' => '60', 'price_JPY' => '10180', 'price_CNY' => '', 'price_AUD' => '162', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydro-tubes-50-pc', 'meta_title' => 'Hydro Tubes', 'meta_keywords' => '', 'meta_description' => 'Hydro Tubes', 'modified' => '2020-04-07 05:19:42', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1824', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup>', 'description' => '<p><span>The Megaruptor</span><sup>®</sup><span> was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA from </span><strong>2 kb - 75 kb</strong><span>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p>', 'label1' => 'Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores', 'info1' => '<h6><img src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" width="851" height="245" /></h6> <h6 style="text-align: center;">Validation using two different DNA sources and two different methods of analysis. <strong>A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 2, 3, 5 and 8 kb. (Standard Sensitivity Large Fragment Analysis Kit ; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010001', 'old_catalog_number' => '', 'sf_code' => null, 'type' => 'ACC', 'search_order' => '04-undefined', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor-1-unit', 'meta_title' => 'Megaruptor', 'meta_keywords' => '', 'meta_description' => 'Megaruptor', 'modified' => '2019-02-20 12:43:01', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2646', 'antibody_id' => null, 'name' => 'Hydropore - long', 'description' => '<p><span style="font-weight: 400;">Hydropore-longは<strong>10 kb - 75 kb</strong>の再現性のあるDNA断片化を可能にします。HydroporesはMegaruptor®（B06010001）と併用する特別設計になっています。</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '特徴', 'info1' => '<p>Sterile hydropore are intended for single use</p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '10 pc', 'catalog_number' => 'E07010002', 'old_catalog_number' => '', 'sf_code' => 'E07010002-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '145', 'price_USD' => '170', 'price_GBP' => '130', 'price_JPY' => '22715', 'price_CNY' => '', 'price_AUD' => '425', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydropore-long-10-pc', 'meta_title' => 'Hydropore - long', 'meta_keywords' => '', 'meta_description' => 'Hydropore - long', 'modified' => '2022-04-18 09:02:42', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2645', 'antibody_id' => null, 'name' => 'Hydropore - short', 'description' => '<p><span style="font-weight: 400;">Hydropore-shortは<strong>2 kb - 9 kb</strong>の再現性のあるDNA断片化を可能にします。HydroporesはMegaruptor®（B06010001）と併用する特別設計になっています。</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '特徴', 'info1' => '<p>Sterile hydropore are intended for single use</p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '10 pc', 'catalog_number' => 'E07010001', 'old_catalog_number' => '', 'sf_code' => 'E07010001-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '135', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '21150', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydropore-short-10-pc', 'meta_title' => 'Hydropore - short', 'meta_keywords' => '', 'meta_description' => 'Hydropore - short', 'modified' => '2022-04-18 09:01:09', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2829', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 2', 'description' => '<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p> <p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>', 'label1' => '', 'info1' => '', 'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2', 'info2' => '<h6><img alt="DNA Shearing Device" style="display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig2.png" width="851" height="267" /></h6> <h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6> <h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010002', 'old_catalog_number' => '', 'sf_code' => 'B06010002-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '17500', 'price_USD' => '19000', 'price_GBP' => '14500', 'price_JPY' => '2741375', 'price_CNY' => '', 'price_AUD' => '47500', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor2-1-unit', 'meta_title' => 'Megaruptor 2', 'meta_keywords' => '', 'meta_description' => 'Megaruptor 2', 'modified' => '2021-05-28 10:49:08', 'created' => '2016-08-30 16:32:45', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '783', 'name' => 'Megaruptor® DNA Shearing System User Manual v2', 'description' => '<div class="page" title="Page 4"> <div class="layoutArea"> <div class="column"> <p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/megaruptor/Megaruptor_manual.pdf', 'slug' => 'megaruptor-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-23 17:29:32', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '118', 'name' => 'product/shearing_technologies/C30010018 _hydro_tubes.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/megaruptor-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor.jpg" alt="megaruptor" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor®</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-long-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2646" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2646" id="CartAdd/2646Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2646" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - long個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hydropore-long-10-pc" data-reveal-id="cartModal-2646" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Hydropore - long</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-short-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2645" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2645" id="CartAdd/2645Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2645" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - 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(High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6> <h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010002', 'old_catalog_number' => '', 'sf_code' => 'B06010002-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '17500', 'price_USD' => '19000', 'price_GBP' => '14500', 'price_JPY' => '2741375', 'price_CNY' => '', 'price_AUD' => '47500', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor2-1-unit', 'meta_title' => 'Megaruptor 2', 'meta_keywords' => '', 'meta_description' => 'Megaruptor 2', 'modified' => '2021-05-28 10:49:08', 'created' => '2016-08-30 16:32:45', 'ProductsRelated' => array( 'id' => '4370', 'product_id' => '2647', 'related_id' => '2829' ), 'Image' => array( (int) 0 => array( 'id' => '1745', 'name' => 'product/shearing_technologies/B06010001_megaruptor2.jpg', 'alt' => ' DNA Shearing, RNA shearing and Chromatin shearing', 'modified' => '2020-11-27 06:56:56', 'created' => '2016-08-30 16:30:47', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '783', 'name' => 'Megaruptor® DNA Shearing System User Manual v2', 'description' => '<div class="page" title="Page 4"> <div class="layoutArea"> <div class="column"> <p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/megaruptor/Megaruptor_manual.pdf', 'slug' => 'megaruptor-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-23 17:29:32', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '565', 'product_id' => '2647', 'document_id' => '783' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p>', 'label1' => 'Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores', 'info1' => '<h6><img src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" width="851" height="245" /></h6> <h6 style="text-align: center;">Validation using two different DNA sources and two different methods of analysis. <strong>A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 2, 3, 5 and 8 kb. (Standard Sensitivity Large Fragment Analysis Kit ; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010001', 'old_catalog_number' => '', 'sf_code' => null, 'type' => 'ACC', 'search_order' => '04-undefined', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor-1-unit', 'meta_title' => 'Megaruptor', 'meta_keywords' => '', 'meta_description' => 'Megaruptor', 'modified' => '2019-02-20 12:43:01', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2646', 'antibody_id' => null, 'name' => 'Hydropore - long', 'description' => '<p><span style="font-weight: 400;">Hydropore-longは<strong>10 kb - 75 kb</strong>の再現性のあるDNA断片化を可能にします。HydroporesはMegaruptor®（B06010001）と併用する特別設計になっています。</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '特徴', 'info1' => '<p>Sterile hydropore are intended for single use</p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '10 pc', 'catalog_number' => 'E07010002', 'old_catalog_number' => '', 'sf_code' => 'E07010002-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '145', 'price_USD' => '170', 'price_GBP' => '130', 'price_JPY' => '22715', 'price_CNY' => '', 'price_AUD' => '425', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydropore-long-10-pc', 'meta_title' => 'Hydropore - long', 'meta_keywords' => '', 'meta_description' => 'Hydropore - long', 'modified' => '2022-04-18 09:02:42', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2645', 'antibody_id' => null, 'name' => 'Hydropore - short', 'description' => '<p><span style="font-weight: 400;">Hydropore-shortは<strong>2 kb - 9 kb</strong>の再現性のあるDNA断片化を可能にします。HydroporesはMegaruptor®（B06010001）と併用する特別設計になっています。</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '特徴', 'info1' => '<p>Sterile hydropore are intended for single use</p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '10 pc', 'catalog_number' => 'E07010001', 'old_catalog_number' => '', 'sf_code' => 'E07010001-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '135', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '21150', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydropore-short-10-pc', 'meta_title' => 'Hydropore - short', 'meta_keywords' => '', 'meta_description' => 'Hydropore - short', 'modified' => '2022-04-18 09:01:09', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2829', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 2', 'description' => '<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p> <p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>', 'label1' => '', 'info1' => '', 'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2', 'info2' => '<h6><img alt="DNA Shearing Device" style="display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig2.png" width="851" height="267" /></h6> <h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6> <h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010002', 'old_catalog_number' => '', 'sf_code' => 'B06010002-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '17500', 'price_USD' => '19000', 'price_GBP' => '14500', 'price_JPY' => '2741375', 'price_CNY' => '', 'price_AUD' => '47500', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor2-1-unit', 'meta_title' => 'Megaruptor 2', 'meta_keywords' => '', 'meta_description' => 'Megaruptor 2', 'modified' => '2021-05-28 10:49:08', 'created' => '2016-08-30 16:32:45', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '783', 'name' => 'Megaruptor® DNA Shearing System User Manual v2', 'description' => '<div class="page" title="Page 4"> <div class="layoutArea"> <div class="column"> <p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/megaruptor/Megaruptor_manual.pdf', 'slug' => 'megaruptor-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-23 17:29:32', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '118', 'name' => 'product/shearing_technologies/C30010018 _hydro_tubes.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/megaruptor-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor.jpg" alt="megaruptor" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor®</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-long-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2646" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2646" id="CartAdd/2646Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2646" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - long個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hydropore-long-10-pc" data-reveal-id="cartModal-2646" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Hydropore - long</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-short-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2645" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2645" id="CartAdd/2645Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2645" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - short個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - short', 'E07010001', '160', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - short', 'E07010001', '160', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hydropore-short-10-pc" data-reveal-id="cartModal-2645" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Hydropore - short</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/megaruptor2-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt=" DNA Shearing, RNA shearing and Chromatin shearing" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2829" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 2</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2829" id="Quote-2829" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2829" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="U3pkYUJKZFdKcDVhcHJxZEdkYnVzdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="megaruptor2-1-unit" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2829"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option value="AW">Aruba</option> <option value="AU">Australia</option> <option value="AT">Austria</option> <option value="AZ">Azerbaijan</option> <option value="BS">Bahamas</option> <option value="BH">Bahrain</option> <option value="BD">Bangladesh</option> <option value="BB">Barbados</option> <option value="BY">Belarus</option> <option value="BE">Belgium</option> <option value="BZ">Belize</option> <option value="BJ">Benin</option> <option value="BM">Bermuda</option> <option value="BT">Bhutan</option> <option value="BO">Bolivia</option> <option value="BQ">Bonaire, Sint Eustatius and Saba</option> <option value="BA">Bosnia and Herzegovina</option> <option value="BW">Botswana</option> <option value="BV">Bouvet Island</option> <option value="BR">Brazil</option> <option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option value="CU">Cuba</option> <option value="CW">Curaçao</option> <option value="CY">Cyprus</option> <option value="CZ">Czech Republic</option> <option value="DK">Denmark</option> <option value="DJ">Djibouti</option> <option value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option value="MP">Northern Mariana Islands</option> <option value="NO">Norway</option> <option value="OM">Oman</option> <option value="PK">Pakistan</option> <option value="PW">Palau</option> <option value="PS">Palestine, State of</option> <option value="PA">Panama</option> <option value="PG">Papua New Guinea</option> <option value="PY">Paraguay</option> <option value="PE">Peru</option> <option value="PH">Philippines</option> <option value="PN">Pitcairn</option> <option value="PL">Poland</option> <option value="PT">Portugal</option> <option value="PR">Puerto Rico</option> <option value="QA">Qatar</option> <option value="RE">Réunion</option> <option value="RO">Romania</option> <option value="RU">Russian Federation</option> <option value="RW">Rwanda</option> <option value="BL">Saint Barthélemy</option> <option value="SH">Saint Helena, Ascension and Tristan da Cunha</option> <option value="KN">Saint Kitts and Nevis</option> <option value="LC">Saint Lucia</option> <option value="MF">Saint Martin (French part)</option> <option value="PM">Saint Pierre and Miquelon</option> <option value="VC">Saint Vincent and the Grenadines</option> <option value="WS">Samoa</option> <option value="SM">San Marino</option> <option value="ST">Sao Tome and Principe</option> <option value="SA">Saudi Arabia</option> <option value="SN">Senegal</option> <option value="RS">Serbia</option> <option value="SC">Seychelles</option> <option value="SL">Sierra Leone</option> <option value="SG">Singapore</option> <option value="SX">Sint Maarten (Dutch part)</option> <option value="SK">Slovakia</option> <option value="SI">Slovenia</option> <option value="SB">Solomon Islands</option> <option value="SO">Somalia</option> <option value="ZA">South Africa</option> <option value="GS">South Georgia and the South Sandwich Islands</option> <option value="ES">Spain</option> <option value="LK">Sri Lanka</option> <option value="SD">Sudan</option> <option value="SR">Suriname</option> <option value="SS">South Sudan</option> <option value="SJ">Svalbard and Jan Mayen</option> <option value="SZ">Swaziland</option> <option value="SE">Sweden</option> <option value="CH">Switzerland</option> <option value="SY">Syrian Arab Republic</option> <option value="TW">Taiwan</option> <option value="TJ">Tajikistan</option> <option value="TZ">Tanzania</option> <option value="TH">Thailand</option> <option value="TL">Timor-Leste</option> <option value="TG">Togo</option> <option value="TK">Tokelau</option> <option value="TO">Tonga</option> <option value="TT">Trinidad and Tobago</option> <option value="TN">Tunisia</option> <option value="TR">Turkey</option> <option value="TM">Turkmenistan</option> <option value="TC">Turks and Caicos Islands</option> <option value="TV">Tuvalu</option> <option value="UG">Uganda</option> <option value="UA">Ukraine</option> <option value="AE">United Arab Emirates</option> <option value="GB">United Kingdom</option> <option value="US" selected="selected">United States</option> <option value="UM">United States Minor Outlying Islands</option> <option value="UY">Uruguay</option> <option value="UZ">Uzbekistan</option> <option value="VU">Vanuatu</option> <option value="VE">Venezuela</option> <option value="VN">Viet Nam</option> <option value="VG">Virgin Islands, British</option> <option value="VI">Virgin Islands, U.S.</option> <option value="WF">Wallis and Futuna</option> <option value="EH">Western Sahara</option> <option value="YE">Yemen</option> <option value="ZM">Zambia</option> <option value="ZW">Zimbabwe</option> </select><script> $('#country_selector_quote-2829').selectize(); </script><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">State</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][state]" id="state-2829" maxlength="3" type="text"/><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div> </div> <div class="row collapse" id="email_v"> <div class="small-3 large-2 columns"> <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Comment</span> </div> <div class="small-9 large-10 columns"> <textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha66228902824bd"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-2829" class="alert button expand" form="Quote-2829" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>'); $('#Quote-2829').attr('action','javascript:postToPardot(\'' + $('#Quote-2829').attr('action') + '\', 2829)'); }); $("#Quote-2829 #submit_btn-2829").click(function (e) { if($(this).closest('form')[0].checkValidity()){ e.preventDefault(); //disable the submit button $("#Quote-2829 #submit_btn-2829").attr("disabled", true); $("#Quote-2829 #submit_btn-2829").html("Processing..."); //submit the form $("#Quote-2829").submit(); } }) </script> <script> if ($("#Quote-2829 #country_selector_quote-2829.selectized").val() == 'US') { var val = $("#state-2829").val(); $("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option 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(NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>'); if (val.length == 2) { $("#state-2829").val(val); } $("#state-2829").parent().parent().show(); } else if ($("#Quote-2829 #country_selector_quote-2829.selectized").val() == 'DE') { var val = $("#state-2829").val(); $("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); if (val.length == 2) { $("#state-2829").val(val); } $("#state-2829").parent().parent().show(); } else { $("#Quote-2829 #state-2829").parent().parent().hide(); $("#Quote-2829 #state-2829").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2829" value="">'); } $("#Quote-2829 #country_selector_quote-2829.selectized").change(function() { if (this.value == 'US') { $("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>'); $("#Quote-2829 #state-2829").parent().parent().show(); } else if (this.value == 'CA') { $("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>'); $("#Quote-2829 #state-2829").parent().parent().show(); } else if (this.value == 'DE') { $("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-2829 #state-2829").parent().parent().show(); } else { $("#Quote-2829 #state-2829").parent().parent().hide(); $("#Quote-2829 #state-2829").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2829" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="megaruptor2-1-unit" data-reveal-id="quoteModal-2829" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor® 2</h6> </div> </div> </li> ' $related = array( 'id' => '2829', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 2', 'description' => '<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p> <p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>', 'label1' => '', 'info1' => '', 'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2', 'info2' => '<h6><img alt="DNA Shearing Device" style="display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig2.png" width="851" height="267" /></h6> <h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. 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(Standard Sensitivity Large Fragment Analysis Kit ; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010001', 'old_catalog_number' => '', 'sf_code' => null, 'type' => 'ACC', 'search_order' => '04-undefined', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor-1-unit', 'meta_title' => 'Megaruptor', 'meta_keywords' => '', 'meta_description' => 'Megaruptor', 'modified' => '2019-02-20 12:43:01', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2646', 'antibody_id' => null, 'name' => 'Hydropore - long', 'description' => '<p><span style="font-weight: 400;">Hydropore-longは<strong>10 kb - 75 kb</strong>の再現性のあるDNA断片化を可能にします。HydroporesはMegaruptor®（B06010001）と併用する特別設計になっています。</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '特徴', 'info1' => '<p>Sterile hydropore are intended for single use</p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. 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Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '10 pc', 'catalog_number' => 'E07010001', 'old_catalog_number' => '', 'sf_code' => 'E07010001-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '135', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '21150', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydropore-short-10-pc', 'meta_title' => 'Hydropore - short', 'meta_keywords' => '', 'meta_description' => 'Hydropore - short', 'modified' => '2022-04-18 09:01:09', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2829', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 2', 'description' => '<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p> <p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>', 'label1' => '', 'info1' => '', 'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2', 'info2' => '<h6><img alt="DNA Shearing Device" style="display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig2.png" width="851" height="267" /></h6> <h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6> <h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010002', 'old_catalog_number' => '', 'sf_code' => 'B06010002-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '17500', 'price_USD' => '19000', 'price_GBP' => '14500', 'price_JPY' => '2741375', 'price_CNY' => '', 'price_AUD' => '47500', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor2-1-unit', 'meta_title' => 'Megaruptor 2', 'meta_keywords' => '', 'meta_description' => 'Megaruptor 2', 'modified' => '2021-05-28 10:49:08', 'created' => '2016-08-30 16:32:45', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '783', 'name' => 'Megaruptor® DNA Shearing System User Manual v2', 'description' => '<div class="page" title="Page 4"> <div class="layoutArea"> <div class="column"> <p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/megaruptor/Megaruptor_manual.pdf', 'slug' => 'megaruptor-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-23 17:29:32', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '118', 'name' => 'product/shearing_technologies/C30010018 _hydro_tubes.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/megaruptor-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor.jpg" alt="megaruptor" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor®</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-long-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2646" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2646" id="CartAdd/2646Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2646" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - long個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hydropore-long-10-pc" data-reveal-id="cartModal-2646" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Hydropore - long</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-short-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2645" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2645" id="CartAdd/2645Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2645" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - short個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - short', 'E07010001', '160', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - short', 'E07010001', '160', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hydropore-short-10-pc" data-reveal-id="cartModal-2645" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Hydropore - short</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/megaruptor2-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt=" DNA Shearing, RNA shearing and Chromatin shearing" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2829" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 2</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2829" id="Quote-2829" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2829" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="U3pkYUJKZFdKcDVhcHJxZEdkYnVzdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="megaruptor2-1-unit" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2829"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option 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value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option value="MP">Northern Mariana Islands</option> <option value="NO">Norway</option> <option value="OM">Oman</option> <option value="PK">Pakistan</option> <option value="PW">Palau</option> <option value="PS">Palestine, State of</option> <option value="PA">Panama</option> <option value="PG">Papua New Guinea</option> <option value="PY">Paraguay</option> <option value="PE">Peru</option> <option value="PH">Philippines</option> <option value="PN">Pitcairn</option> <option value="PL">Poland</option> <option value="PT">Portugal</option> <option value="PR">Puerto Rico</option> <option value="QA">Qatar</option> <option value="RE">Réunion</option> <option value="RO">Romania</option> <option value="RU">Russian Federation</option> <option value="RW">Rwanda</option> <option value="BL">Saint Barthélemy</option> <option value="SH">Saint Helena, Ascension and Tristan da Cunha</option> <option value="KN">Saint Kitts and Nevis</option> <option value="LC">Saint Lucia</option> <option value="MF">Saint Martin (French part)</option> <option value="PM">Saint Pierre and Miquelon</option> <option value="VC">Saint Vincent and the Grenadines</option> <option value="WS">Samoa</option> <option value="SM">San Marino</option> <option value="ST">Sao Tome and Principe</option> <option value="SA">Saudi Arabia</option> <option value="SN">Senegal</option> <option value="RS">Serbia</option> <option value="SC">Seychelles</option> <option value="SL">Sierra Leone</option> <option value="SG">Singapore</option> <option value="SX">Sint Maarten (Dutch part)</option> <option value="SK">Slovakia</option> <option value="SI">Slovenia</option> <option value="SB">Solomon Islands</option> <option value="SO">Somalia</option> <option value="ZA">South Africa</option> <option value="GS">South Georgia and the South Sandwich Islands</option> <option value="ES">Spain</option> <option value="LK">Sri Lanka</option> <option value="SD">Sudan</option> <option value="SR">Suriname</option> <option value="SS">South Sudan</option> <option value="SJ">Svalbard and Jan Mayen</option> <option value="SZ">Swaziland</option> <option value="SE">Sweden</option> <option value="CH">Switzerland</option> <option value="SY">Syrian Arab Republic</option> <option value="TW">Taiwan</option> <option value="TJ">Tajikistan</option> <option value="TZ">Tanzania</option> <option value="TH">Thailand</option> <option value="TL">Timor-Leste</option> <option value="TG">Togo</option> <option value="TK">Tokelau</option> <option value="TO">Tonga</option> <option value="TT">Trinidad and Tobago</option> <option value="TN">Tunisia</option> <option value="TR">Turkey</option> <option value="TM">Turkmenistan</option> <option value="TC">Turks and Caicos Islands</option> <option value="TV">Tuvalu</option> <option 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/></a></p> <p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>', 'label1' => '', 'info1' => '', 'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2', 'info2' => '<h6><img alt="DNA Shearing Device" style="display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig2.png" width="851" height="267" /></h6> <h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6> <h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010002', 'old_catalog_number' => '', 'sf_code' => 'B06010002-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '17500', 'price_USD' => '19000', 'price_GBP' => '14500', 'price_JPY' => '2741375', 'price_CNY' => '', 'price_AUD' => '47500', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor2-1-unit', 'meta_title' => 'Megaruptor 2', 'meta_keywords' => '', 'meta_description' => 'Megaruptor 2', 'modified' => '2021-05-28 10:49:08', 'created' => '2016-08-30 16:32:45', 'ProductsRelated' => array( 'id' => '4370', 'product_id' => '2647', 'related_id' => '2829' ), 'Image' => array( (int) 0 => array( 'id' => '1745', 'name' => 'product/shearing_technologies/B06010001_megaruptor2.jpg', 'alt' => ' DNA Shearing, RNA shearing and Chromatin shearing', 'modified' => '2020-11-27 06:56:56', 'created' => '2016-08-30 16:30:47', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '783', 'name' => 'Megaruptor® DNA Shearing System User Manual v2', 'description' => '<div class="page" title="Page 4"> <div class="layoutArea"> <div class="column"> <p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/megaruptor/Megaruptor_manual.pdf', 'slug' => 'megaruptor-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-23 17:29:32', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '565', 'product_id' => '2647', 'document_id' => '783' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p>', 'label1' => 'Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores', 'info1' => '<h6><img src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" width="851" height="245" /></h6> <h6 style="text-align: center;">Validation using two different DNA sources and two different methods of analysis. <strong>A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 2, 3, 5 and 8 kb. (Standard Sensitivity Large Fragment Analysis Kit ; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010001', 'old_catalog_number' => '', 'sf_code' => null, 'type' => 'ACC', 'search_order' => '04-undefined', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor-1-unit', 'meta_title' => 'Megaruptor', 'meta_keywords' => '', 'meta_description' => 'Megaruptor', 'modified' => '2019-02-20 12:43:01', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2646', 'antibody_id' => null, 'name' => 'Hydropore - long', 'description' => '<p><span style="font-weight: 400;">Hydropore-longは<strong>10 kb - 75 kb</strong>の再現性のあるDNA断片化を可能にします。HydroporesはMegaruptor®（B06010001）と併用する特別設計になっています。</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '特徴', 'info1' => '<p>Sterile hydropore are intended for single use</p> <p><strong>Storage</strong><br />Store at room temperature</p> <p><strong>Precautions</strong><br />This product is for research use only. 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Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '10 pc', 'catalog_number' => 'E07010001', 'old_catalog_number' => '', 'sf_code' => 'E07010001-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '135', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '21150', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hydropore-short-10-pc', 'meta_title' => 'Hydropore - short', 'meta_keywords' => '', 'meta_description' => 'Hydropore - short', 'modified' => '2022-04-18 09:01:09', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2829', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 2', 'description' => '<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p> <p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>', 'label1' => '', 'info1' => '', 'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2', 'info2' => '<h6><img alt="DNA Shearing Device" style="display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig2.png" width="851" height="267" /></h6> <h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6> <h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010002', 'old_catalog_number' => '', 'sf_code' => 'B06010002-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '17500', 'price_USD' => '19000', 'price_GBP' => '14500', 'price_JPY' => '2741375', 'price_CNY' => '', 'price_AUD' => '47500', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor2-1-unit', 'meta_title' => 'Megaruptor 2', 'meta_keywords' => '', 'meta_description' => 'Megaruptor 2', 'modified' => '2021-05-28 10:49:08', 'created' => '2016-08-30 16:32:45', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '783', 'name' => 'Megaruptor® DNA Shearing System User Manual v2', 'description' => '<div class="page" title="Page 4"> <div class="layoutArea"> <div class="column"> <p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/megaruptor/Megaruptor_manual.pdf', 'slug' => 'megaruptor-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-23 17:29:32', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '118', 'name' => 'product/shearing_technologies/C30010018 _hydro_tubes.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/megaruptor-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor.jpg" alt="megaruptor" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor®</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-long-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2646" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2646" id="CartAdd/2646Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2646" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - long個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - long', 'E07010002', '170', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hydropore-long-10-pc" data-reveal-id="cartModal-2646" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Hydropore - long</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/hydropore-short-10-pc"><img src="/img/product/shearing_technologies/hydropores.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07010001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2645" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2645" id="CartAdd/2645Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2645" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Hydropore - short個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - short', 'E07010001', '160', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Hydropore - short', 'E07010001', '160', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hydropore-short-10-pc" data-reveal-id="cartModal-2645" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Hydropore - short</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/megaruptor2-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt=" DNA Shearing, RNA shearing and Chromatin shearing" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2829" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 2</strong>. 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha66228902824bd"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-2829" class="alert button expand" form="Quote-2829" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-2829 #state-2829").parent().parent().show(); } else { $("#Quote-2829 #state-2829").parent().parent().hide(); $("#Quote-2829 #state-2829").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2829" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="megaruptor2-1-unit" data-reveal-id="quoteModal-2829" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor® 2</h6> </div> </div> </li> ' $related = array( 'id' => '2829', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 2', 'description' => '<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p> <p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>', 'label1' => '', 'info1' => '', 'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2', 'info2' => '<h6><img alt="DNA Shearing Device" style="display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/shearing_technologies/megaruptor-fig2.png" width="851" height="267" /></h6> <h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6> <h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B06010002', 'old_catalog_number' => '', 'sf_code' => 'B06010002-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '17500', 'price_USD' => '19000', 'price_GBP' => '14500', 'price_JPY' => '2741375', 'price_CNY' => '', 'price_AUD' => '47500', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'megaruptor2-1-unit', 'meta_title' => 'Megaruptor 2', 'meta_keywords' => '', 'meta_description' => 'Megaruptor 2', 'modified' => '2021-05-28 10:49:08', 'created' => '2016-08-30 16:32:45', 'ProductsRelated' => array( 'id' => '4370', 'product_id' => '2647', 'related_id' => '2829' ), 'Image' => array( (int) 0 => array( 'id' => '1745', 'name' => 'product/shearing_technologies/B06010001_megaruptor2.jpg', 'alt' => ' DNA Shearing, RNA shearing and Chromatin shearing', 'modified' => '2020-11-27 06:56:56', 'created' => '2016-08-30 16:30:47', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '783', 'name' => 'Megaruptor® DNA Shearing System User Manual v2', 'description' => '<div class="page" title="Page 4"> <div class="layoutArea"> <div class="column"> <p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/megaruptor/Megaruptor_manual.pdf', 'slug' => 'megaruptor-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-23 17:29:32', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '565', 'product_id' => '2647', 'document_id' => '783' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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