Figure 2. Western blot analysis using the Diagenode antibody directed against MBD3
Figure 2A: Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 μg) with the Diagenode antibody against human MBD3 (Cat. No. CS-079-100), diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Figure 2B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (diluted 1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). The location of tagged MBD3 is indicated with an arrow.