Diagenode

MBD1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410078
(pAb-078-050)
50 µg/72 µl
$295.00
  Bulk order

Alternative names: CXXC3, PCM1, RFT

Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.

LotA17-0042
Concentration0.7 µg/µl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1.5 μg/ChIP Fig 1
ELISA 1:1,000 Fig 2
Western Blotting 1:500 Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP

    Figure 1 ChIP results obtained with the Diagenode antibody directed against MBD1
    ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against MBD1 (Cat. No. pAb-078-050) and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 1.5 μg of antibody were used per ChIP experiment. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ELISA

    Figure 2 Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (Cat. No. pAb-078-050), crude serum and Flow Through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the purified antibody was estimated to be 1:20,000.

    Western blot

    Figure 3 Western blot analysis using the Diagenode antibody directed against MBD1
    Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against MBD1 (Cat. No. pAb-078-050) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet MBD1 C15410078 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MBD1 polyclonal antibody - Classic (Diagenode Cat# C15410078 Lot# A17-0042). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    H19 lncRNA controls gene expression of the Imprinted Gene Network by recruiting MBD1.
    Monnier P, Martinet C, Pontis J, Stancheva I, Ait-Si-Ali S, Dandolo L
    The H19 gene controls the expression of several genes within the Imprinted Gene Network (IGN), involved in growth control of the embryo. However, the underlying mechanisms of this control remain elusive. Here, we identified the methyl-CpG-binding domain protein 1 MBD1 as a physical and functional partner of the H19 ...

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