Diagenode

LSD1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410125
(pAb-125-050)
50 µg/75 µl
$295.00
  Bulk order

Alternative names: BHC110, AOF2, EC1, KDM1

Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.

LotA56-001
Concentration0.67 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 Fig 1
Western Blotting 1:1,000 Fig 2, Fig 3
Immunoprecipitation 2 μg/IP Fig 3
  • Validation Data

    ELISA

    Figure 1 Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.

    Western blot

    Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1
    Western blot was performed using respectively 150 μg (lane 1) or 50 μg (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left.

    Immunoprecipitation and Western blot analysis

    Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1
    293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 μg of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  • Documents
    Datasheet LSD1 pAb-125-050 DATASHEET
    Datasheet description
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: LSD1 polyclonal antibody - Classic (Diagenode Cat# C15410125 Lot# A56-001 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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