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Figure 1 Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.
Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1 Western blot was performed using respectively 150 μg (lane 1) or 50 μg (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left.
Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 μg of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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