Diagenode

HDAC1 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15100144
(SN-144-100)
100 µl
$295.00
  Bulk order

Alternative names: HD1, RPD3, RPD3L1, GON-10

Monoclonal antibody raised in mouse against human HDAC1 (Histone deacetylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.

Lot001
Concentrationnot determined
Species reactivityHuman
TypeMonoclonal
PurityConcentrated supernatant
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 3 μl/ChIP Fig 1
Western Blotting 1:1,000 Fig 2

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against HDAC1
    ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against HDAC1 (Cat. No. C15100144) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (Cat. No. C01010070), using sheared chromatin from 10,000 cells. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the coding region of p21, a known target gene of HDAC1. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Western Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against HDAC1
    Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode monoclonal antibody against HDAC1 (Cat. No. C15100144) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet HDAC1 SN-144-100 DATASHEET
    Datasheet description
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HDAC1 monoclonal antibody - Classic (Diagenode Cat# C15100144 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
  • XXXIX Reunión anual de la Sociedad de Bioquimica y Biología Molecular de Chile
    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
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