Diagenode

HDAC1 monoclonal antibody

目录号
格式
价格
C15100144
(SN-144-100)
100 µl
$380.00
  Bulk order



Alternative names: HD1, RPD3, RPD3L1, GON-10

Monoclonal antibody raised in mouse against human HDAC1 (Histone deacetylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.

Lot001
Concentrationnot determined
Species reactivityHuman
TypeMonoclonal
PurityConcentrated supernatant
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 3 μl/ChIP Fig 1
Western Blotting 1:1,000 Fig 2

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    HDAC1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against HDAC1
    ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against HDAC1 (Cat. No. C15100144) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (Cat. No. C01010070), using sheared chromatin from 10,000 cells. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the coding region of p21, a known target gene of HDAC1. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    HDAC1 Antibody validated in Western Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against HDAC1
    Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode monoclonal antibody against HDAC1 (Cat. No. C15100144) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.

    HDAC1 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against HDAC1
    Whole cell extracts (40 μg) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against HDAC1 (Cat. No. C15100144) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.

  •  应用
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
    siRNA Knockdown
    Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level o... Read more
  •  文档
    Datasheet HDAC1 SN-144-100 DATASHEET
    Datasheet description
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  Safety sheets
    SDS C15200144 HDAC1 Antibody US en Download
    SDS C15200144 HDAC1 Antibody GB en Download
    SDS C15200144 HDAC1 Antibody BE fr Download
    SDS C15200144 HDAC1 Antibody FR fr Download
    SDS C15200144 HDAC1 Antibody ES es Download
    SDS C15200144 HDAC1 Antibody DE de Download
    SDS C15200144 HDAC1 Antibody JP ja Download
    SDS C15200144 HDAC1 Antibody BE nl Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HDAC1 monoclonal antibody (Diagenode Cat# C15100144 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    HDAC1 links early life stress to schizophrenia-like phenotypes
    Bahari-Javan S. et al.
    Schizophrenia is a devastating disease that arises on the background of genetic predisposition and environmental risk factors, such as early life stress (ELS). In this study, we show that ELS-induced schizophrenia-like phenotypes in mice correlate with a widespread increase of histone-deacetylase 1 (Hdac1) expressio...

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