Diagenode

H4R3me1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410299
50 μg
$350.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H4 (monomethyl Arg3), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.43 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunofluorescence 1:50 Figure 1
Western Blotting 1:1,000 Figure 2
Immunochemistry 1:50
Dot Blotting 1:1,000
  • Validation Data

    Immunofluorescence

    Figure 1. Immunofluorescence
    Immunofluorescence of H4R3me1 antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4R3me1 is nuclear and chromosomal. Staining: Histone H4R3me1 is expressed in green, nuclei are counterstained with DAPI (blue).

    Western Blot

    Figure 2. Western Blot
    Western Blot of H4R3me1 antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:1,000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
  • Documents
    Datasheet H4R3me1 C15410299 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4R3me1 polyclonal antibody - Classic (Diagenode Cat# C15410299 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products

Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
 See all events

Twitter feed

News

 See all news