Diagenode

H4K20me2 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200220
50 µg
$295.00
  Bulk order

Monoclonal antibody raised in mouse against histone H4, dimethylated at lysine 20 (H4K20me2), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 µg/µl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-5 μg/ChIP Figure 1
Dot Blotting 1:20,000 Figure 2
Western Blotting 1:1,000 Figure 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H4K20me2
    ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K20me2 (Cat. No. C15200220) and optimized PCR primer sets for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit (Cat. No. C01010022) on the IP-Star automated system, using sheared chromatin from 1 million cells. Respectively 1 and 5 μg of antibody was used per ChIP experiment. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and SatA satellite repeats, used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Dot Blot

    Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody directed against H4K20me2
    To test the cross reactivity of the Diagenode monoclonal antibody against H4K20me2 (Cat. No. C15200220), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    Western Blot

    Figure 3. Western blot analysis using the Diagenode monoclonal directed antibody against H4K20me2
    Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H4K20me2 (Cat. No. C15200220) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    C15200220 H4K20me2 monoclonal DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K20me2 monoclonal antibody - Classic (Diagenode Cat# C15200220 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products

Events

 See all events

Twitter feed

News

 See all news