Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H4K20me2
ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K20me2 (Cat. No. C15200220) and optimized PCR primer sets for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit (Cat. No. C01010022) on the IP-Star automated system, using sheared chromatin from 1 million cells. Respectively 1 and 5 μg of antibody was used per ChIP experiment. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and SatA satellite repeats, used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).