H4K20me2 monoclonal antibody

50 µg
  Bulk order

Monoclonal antibody raised in mouse against histone H4, dimethylated at lysine 20 (H4K20me2), using a KLH-conjugated synthetic peptide.

Concentration1 µg/µl
Species reactivityHuman
PurityProtein A purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-5 μg/ChIP Figure 1
Dot Blotting 1:20,000 Figure 2
Western Blotting 1:1,000 Figure 3
Immunofluorescence 1:1,000 Figure 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    H4K20me2 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H4K20me2
    ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K20me2 (Cat. No. C15200220) and optimized PCR primer sets for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit (Cat. No. C01010022) on the IP-Star automated system, using sheared chromatin from 1 million cells. Respectively 1 and 5 μg of antibody was used per ChIP experiment. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and SatA satellite repeats, used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    H4K20me2 Antibody validated in Dot Blot

    Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody directed against H4K20me2
    To test the cross reactivity of the Diagenode monoclonal antibody against H4K20me2 (Cat. No. C15200220), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    H4K20me2 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode monoclonal directed antibody against H4K20me2
    Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H4K20me2 (Cat. No. C15200220) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    H4K20me2 Antibody validated in Immunofluorescence

    Figure4. Immunofluorescence using the Diagenode monoclonal antibody directed against H4K20me2
    HeLa cells were stained with the Diagenode monoclonal antibody against H4K20me2 (Cat. No. C15200220) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H4K20me2 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of H4K20 is associated with inactive genomic regions.

  •  应用
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Dot blotting Read more
  •  文档
    C15200220 H4K20me2 monoclonal DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    H4K20me2 antibody SDS GB en Download
    H4K20me2 antibody SDS US en Download
    H4K20me2 antibody SDS BE nl Download
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  •  出版物

    How to properly cite this product in your work

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