Diagenode

H3T6pK9me3 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410285
50 μg
$350.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (p Thr6), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.55 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunohistochemistry 1:200
Immunofluorescence 1:100 Fig 1
Western Blotting 1:500 Fig 2, 3
Dot Blotting 1:1,000 Fig 4
  • Validation Data

    ChIP results

    Figure 1. H3T6pK9me3 antibody ChIP results
    Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin.

    Immunofluorescence results

    Figure 2 H3T6pK9me3 antibody Immunofluorescence results
    Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue).

    Western blot results

    Figure 3. H3T6pK9me3 antibody Western blot results
    Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Western blot results

    Figure 4. H3T6pK9me3 antibody Western blot results
    Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot blot results

    Figure 5. H3T6pK9me3 antibody Dot blot results
    Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  • Applications
    IHC
    Immunohistochemistry Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H3T6p C15410282 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

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