Diagenode

H3T6p polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410282
50 μg
$350.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (p Thr6), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.67 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunohistochemistry 1:200
Immunofluorescence 1:100 Figure 1
Western Blotting 1:500 Figure 2
Dot Blotting 1:1,000 Figure 3
  • Validation Data

    IF results

    Figure 1. H3T6p antibody IF results
    Immunofluorescence of H3T6p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T6p is nuclear and chromosomal. Staining: H3T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

    Western blot results

    Figure 2. H3T6p antibody Western blot results
    Western Blot of H3T6p antibody. 30 μg C. elegans embryo lysate. Primary antibody incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Western blot results

    Figure 3. H3T6p antibody Western blot results
    Western Blot of H3T6p antibody on 30 μg HeLa histone extracts. Primary antibody: incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot blot results

    Figure 4. H3T6p antibody dot blot results
    Dot Blot of H3T6p antibody. Lane 1: H3T6un. Lane 2: H3T6p. Load: 1, 0.3, 0.1, 0.03 picomoles of peptide. Primary antibody used at a 1:1000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  • Applications
    IHC
    Immunohistochemistry Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
  • Documents
    Datasheet H3T6p C15410282 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3T6p polyclonal antibody - Classic (Diagenode Cat# C15410282 Lot# 001). Click here to copy to clipboard.

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