Diagenode

H3S10pT11p polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410288
50 μg
$350.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (p Ser10, p Thr11), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1.2 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunohistochemistry 1:200
Immunofluorescence 1:200 Figure 1
Western Blotting 1:500 Figure 2, 3
  • Validation Data

    Immunofluorescence results

    Figure 1. H3S10pT11p Immunofluorescence results
    Immunofluorescence of H3S10pT11p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: H3S10pT11p antibody at a 1:200 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3S10pT11p is nuclear and chromosomal. Staining: H3S10pT11p is expressed in green, nuclei are counterstained with Dapi (blue).

    Western blot results

    Figure 2 H3S10pT11p Western blot results
    Western Blot of H3S10pT11p antibody. 30 μg C. elegans embryo lysate. Primary antibody: H3S10pT11p at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Western blot results

    Figure 3. H3S10pT11p Western blot results
    Western Blot of H3S10pT11p antibody. 30 μg HeLa histone extracts. Primary antibody: H3S10pT11p at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

  • Applications
    IHC
    Immunohistochemistry Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet H3S10pT11p C15410288 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

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