Diagenode

H3K79ac monoclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15210006
100 µg /100 µl
$295.00
  Bulk order

Monoclonal antibody raised in rabbit against histone H3 acetylated at Lys79 (H3K79ac), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified
HostRabbit
StorageStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μg/ChIP Fig 1, 2
Western blot 1:2,000 Fig 3
Immunofluorescence 1:500 Fig 4
  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K79ac
    ChIP assays were performed on sheared chromatin from HeLa cells using 5 μg of the Diagenode monoclonal antibody against H3K79ac (cat. No. C15210006). QPCR was performed with primers for the coding region of the RPL30 gene, used as positive control and for the inactive MYOD1 gene and the Sata satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq

    ChIP-seq

    ChIP-seq

    ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3K79ac
    ChIP was performed on sheared chromatin from 1 million HeLa cells using 5 μg of the Diagenode antibody against H3K79ac (Cat. No. C15210006) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of human chromosome 3 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and GAPDH positive control genes (fig 2C and D).

    Western blot

    Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3K79ac
    Histone extracts from HeLa cells treated with butyrate (lane 2) or untreated control cells (lane 1) were analysed by Western blot using the Diagenode monoclonal antibody against H3K79ac (cat. No. C15210006) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunofluorescence

    Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K79ac
    HeLa cells treated with butyrate were stained with the Diagenode antibody against H3K79ac No. C15210006 (red) diluted 1:500. Actin filaments were stained with fluorescein phalladoin (green).

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
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    ChIP-seq (ab)
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  • Documents
    H3K79ac monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against histone H3 acetylated at Lys79 (H3K79ac), using a KL...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K79ac monoclonal antibody - Classic (Diagenode Cat# C15210006 Lot# 001). Click here to copy to clipboard.

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