Diagenode

H3K56me3 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410297
50 μg
$350.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (trimethyl Lys56), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.71 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunofluorescence 1:100 Fig 1, 2
Western Blotting 1:500 - 1:1,000 Fig 3
Dot Blotting 1:40 Fig 4
  • Validation Data

    Immunofluorescence

    Figure 1. Immunofluorescence
    Immunofluorescence using the H3K56me3 antibody. Tissue: HeLa cells during telophase. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K56me3 is nuclear and chromosomal. Staining: Histone H3K56me3 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

    Immunofluorescence

    Figure 2. Immunofluorescence
    Immunofluorescence using the H3K56me3 antibody. Tissue: HeLa cells during prophase. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K56me3 is nuclear and chromosomal. Staining: Histone H3K56me3 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

    Western Blot

    Figure 3. Western Blot
    Western Blot using the H3K56me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot Blot

    Figure 4. Dot Blot
    Dot Blot using the H3K56me3 antibody. Lane 1: H3K56ac. Lane 2: H3K56me1. Lane 3: H3K56me2. Lane 4: H3K56me3. Lane 5: H3K56 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:40 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
  • Documents
    Datasheet H3K56me3 C15410297 DATASHEET
    Datasheet description
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  • Publications

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