Diagenode

H3K4me1T6p polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410280
50 μg
$350.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (monomethyl Lys4, p Thr6), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.55 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunohistochemistry 1:50
Immunofluorescence 1:50 Fig 1
Western Blotting 1:500 Fig 2, 3
Dot Blotting 1:1,000 Fig 4
  • Validation Data

    Immunofluorescence

    Figure 1. H3K4me1T6p antibody Immunofluorescence results
    Immunofluorescence of H3K4me1T6p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K4me1T6p is nuclear and chromosomal. Staining: H3K4me1T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

    Western blot

    Figure 2. H3K4me1T6p antibody Western blot results
    Western Blot of Rabbit H3K4me1T6p antibody. 30 μg of C. elegans embryonic lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Western blot

    Figure 3. H3K4me1T6p antibody Western blot results
    Western Blot of H3K4me1T6p antibody. 30 μg of HeLa histone extracts per lane. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot blot results

    Figure 4. H3K4me1T6p antibody Dot blot results
    Dot Blot of H3K4me1T6p antibody. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  • Applications
    IHC
    Immunohistochemistry Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
  • Documents
    Datasheet H3K4me1T6p C15410280 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

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