H2A.XS139p polyclonal antibody - Classic

Catalog Number
50 μg/ 19 μl
  Bulk order

Polyclonal antibody raised in rabbit against the region of histone H2A.X containing the phosphorylated serine 139 (H2A.XS139p), using a KLH-conjugated synthetic peptide.

Concentration2.75 µg/µl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:10,000 Fig 1
Dot Blotting 1:10,000 Fig 2
Western Blotting 1:1,000 Fig 3
Immunofluorescence 1:500 Fig 4
  • Validation Data


    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H2A.XS139p (Cat. No. C15410219) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:170,000.

    Dot Blot

    Figure 2. Cross reactivity tests using the Diagenode antibody directed against H2A.XS139p
    To test the cross reactivity of the Diagenode antibody against H2A. XS139p (Cat. No. C15410219), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2A.X. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    Western blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against H2A.XS139p
    Western blot was performed on histone extracts (15 μg) from untreated U2OS cells (lane 1) or from U2OS cells treated with camptothecin (lane 2), and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H2A.XS139p (Cat. No. C15410219). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.


    Figure 4. Immunofluorescence using the Diagenode antibody directed against H2A.XS139p
    U2OS cells, either treated with camptothecin (figure 4B) or untreated (figure 4A), were stained with the Diagenode antibody against H2A.XS139p (cat. No. C15410219) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H2A.XS139p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Dot blotting Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet H2A.XS139p C15410219 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

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