Diagenode

Flp polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310169
(CS-169-100)
100 µl
$195.00
  Bulk order

 Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.

LotA280-004
Concentrationnot determined
Species reactivity
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:50 Fig 1
Western Blotting 1:500 Fig 2, (1)
  • Validation data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Flp
    Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet Flp CS-169-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Flp polyclonal antibody - Pioneer (Diagenode Cat# C15310169 Lot# A280-004). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity.
    Neshati Z, Liu J, Zhou G, Schalij MJ, de Vries AA
    Cell-to-cell fusion can be quantified by endowing acceptor and donor cells with latent reporter genes/proteins and activators of these genes/proteins, respectively. One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are ...

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