Diagenode

EHMT1 Antibody

Catalog Number
Format
Price
C15310114
(CS-114-100)
100 µl
$260.00
  Bulk order



Alternative names: GLP1, KMT1D

Polyclonal antibody raised in rabbit against mouse Ehmt1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein.

LotA265-004
Concentrationnot determined
Species reactivityHuman, mouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 – 1:200 Fig 1
Western Blotting 1:500 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2c (Cat. No. CS-105-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2c
    Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2c (Cat. No. CS-105-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.

  •  Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    Datasheet Ehmt1 CS-114-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  Safety sheets
    EHMT1 antibody SDS US en Download
    EHMT1 antibody SDS GB en Download
    EHMT1 antibody SDS BE fr Download
    EHMT1 antibody SDS FR fr Download
    EHMT1 antibody SDS ES es Download
    EHMT1 antibody SDS DE de Download
    EHMT1 antibody SDS JP ja Download
    EHMT1 antibody SDS BE nl Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EHMT1 Antibody (Diagenode Cat# C15310114 Lot# A265-004 ). Click here to copy to clipboard.

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