CHD5 polyclonal antibody - Pioneer

Catalog Number
100 µl
  Bulk order

Polyclonal antibody raised in rabbit against human CHD5 (chromodomain helicase DNA binding protein 5), using two KLH-conjugated synthetic peptides containing a sequence from the N-terminus and from the central part of the protein, respectively.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:500 - 1:1,000 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data


    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CHD5 (Cat. No. CS-121-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:93,500.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against CHD5
    HeLa cells were transfected with CHD5 and analysed by Western blot. Western blot was performed using 15 to 30 μg nuclear extracts and the Diagenode antibody against human CHD5 (Cat. No. CS-121-100). The antibody was diluted 1:1,000 in TBS-Tween containing 2% milk powder. The position of the protein of interest (expected size: 223 kDa) is indicated on the right; the marker (in kDa) is shown on the left. Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells. Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet CHD5 CS-121-100 DATASHEET
    Datasheet description
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CHD5 polyclonal antibody - Pioneer (Diagenode Cat# C15310121 Lot# A473-002). Click here to copy to clipboard.

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