Diagenode

CCDC101 polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310122
(CS-122-100)
100 µl
$195.00
  Bulk order

Alternative names: SGF29

Polyclonal antibody raised in rabbit against human CCDC101 (coiled-coil domain containing 101), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively.

LotA509-004
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 – 1:200 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CCDC101 (Cat. No. CS-122-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,700.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against CCDC101
    Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against CCDC101 (Cat. No. CS-122-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 33 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet CCDC101 CS-122-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CCDC101 polyclonal antibody - Pioneer (Diagenode Cat# C15310122 Lot# A509-004 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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