Diagenode

Diagenode One sonication device

Bioruptor One
User friendly software
Tight fragment size distribution
All-in-one desktop device
Integrated temperature control
Sample size 20 µl or 50 µl
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Catalog Number
Format
B01070001
1 unit

The Diagenode One is the new desktop solution that provides optimal DNA shearing for Next-Generation-Sequencing. Designed to fit any bench, it is the smallest and lightest Diagenode shearing device. The incredible fully integrated cooling system and the all-new 20 and 50 µl microfluidic chips have been enhanced to deliver the highest performance. 

  • Compact size
  • Small volume DNA shearing for optimal NGS library prep
  • Fully integrated system

  • Highly reproducible DNA shearing

    The One provide the most consistent and ideal shearing (200 bp - 1 kb) to ensure subsequent high quality NGS data.

    High and homogeneous shearing performance. Fragment AnalyzerTM (Advanced Analytical Technologies, Inc.) profile showing the tight distribution for 8 independent DNA samples prepared with the One.

  • Unbiased DNA shearing for Next-Generation Sequencing

    The One is specifically designed to prepare high-quality sheared DNA prior to create unbiaised libraries with even distribution and representation of the genome. The perfect unbiased shearing enables ultra-high and uniform coverage of libraries prepared from even AT-GC (E. Coli), AT-rich (S. Epidermis) and, GC-rich (B. Pertussis) bacterial genomes. The whole-genome sequencing on the Illumina® platform (Hiseq instrument, 50 base long single end reads) shows an excellent distribution for all three genomes.

    E. Coli genome uniform and consitent coverage. Number of matched reads to the reference genome.

    S. Epidermis genome uniform and consitent coverage. Number of matched reads to the reference genome.

    B. Pertussis genome uniform and consitent coverage. Number of matched reads to the reference genome.

    Unbiaised E. Coli DNA shearing for best coverage. Overall distribution of read depths for an even AT-GC genome.

    Unbiaised S. Epidermis DNA shearing for best coverage. Overall distribution of read depths for a AT-rich genome.

    Unbiaised B. Pertussis DNA shearing for best coverage. Overall distribution of read depths for a CG-rich genome.

  • Applications
    DNA/RNA shearing
    In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critic... Read more
    Next Generation Sequencing
    DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 kb to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA libr... Read more
  • Documents
    Diagenode One manual MANUAL
    Manual for Diagenode One sonication machine.
    Download
    Bioruptor® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Diagenode One sonication device (Diagenode Cat# B01070001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    DNA fragmentation and quality control analysis
    Wassim Lakhal, Irina Panteleeva, Sharon Squazzo, Rini Saxena, Jerome Kroonen, Steve Siembieda, Markus Tilmes & Jonathan Hagopian
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, namely, precise DNA fragmentation, size-range analysis, and smear quantification. The Diagenode One, Bioruptor®, Megaruptor®, and Fragment Analyzer™ by Advanced Analytical ensure that these first cri...

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