Diagenode

Auto MethylCap kit

Product image
Catalog Number
Format
Price
C02020011
(AF-Auto01-0048)
48 rxns
$495.00

The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCapTM kit is designed to perform automated immunoprecipitation of Chromatin using the SX-8G IP-Star® Automated System enabling highly reproducible results and allowing for high throughput.

  • Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a ... Read more
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
    Methylbinding domain protein
    The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containin... Read more
  • Documents
    Auto MethylCap kit MANUAL
    This H6-GST-MBD fusion protein (cat# mbd-001-100) has been extensively validated. It consis...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Auto MethylCap kit (Diagenode Cat# C02020011). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Aberrant DNA methylation of WNT pathway genes in the development and progression of CIMP-negative colorectal cancer.
    Galamb O et al.
    The WNT signaling pathway has an essential role in colorectal carcinogenesis and progression, which involves a cascade of genetic and epigenetic changes. We aimed to analyze DNA methylation affecting the WNT pathway genes in colorectal carcinogenesis in promoter and gene body regions using whole methylome analysis i...

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  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
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    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
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