Diagenode

ASH2 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310093
(CS-093-100)
100 µl
$295.00
  Bulk order

Alternative names: ASH2L, ASH2L1, ASH2L2, Bre2

Polyclonal antibody raised in rabbit against mouse Ash2 (absent, small, or homeotic 2), using 3 different KLH-conjugated synthetic peptides, 2 containing an amino acid sequence from the central and 1 containing an amino acid sequence from the C-terminal part of the protein.

LotA260-004
Concentrationnot determined
Species reactivityMouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:500 Fig 1
Western Blotting 1:500 - 1:1,000 Fig 2
Immunofluorescence 1:200 Fig 3
  • Validation Data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Ash2 (Cat. No. C15310093). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:24,000.

    Immunofluorescence

    Figure 2. Western blot analysis using the Diagenode antibody directed against Ash2
    A. Western blot was performed on whole cell lysates from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Ash2 (Cat. No. C15310093), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the right; the location of the protein of interest (predicted size: 68 kDa) is indicated on the left. B. Western blot was performed on whole cell lysates from mouse neural stem cells (NS), transfected with GFP tagged Ash2, with the Diagenode antibody against mouse Ash2 (Cat. No. C15310093), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the endogenous Ash2 (68 kDa) and of the GFP tagged Ash2 (106 kDa) are indicated on the right.

    Western Blot

    Figure 3. Immunofluorescence using the Diagenode antibody directed against Ash2
    NIH3T3 cells were stained with the Diagenode antibody against Ash2 (Cat. No. C15310093) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Ash2 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet ASH2 CS-093-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ASH2 polyclonal antibody - Classic (Diagenode Cat# C15310093 Lot# A260-004). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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