Diagenode

Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation.


Sandovici I, Nicholas LM, O'Neill LP

The islets of Langerhans are clusters of cells dispersed throughout the pancreas that produce several hormones essential for controlling a variety of metabolic processes, including glucose homeostasis and lipid metabolism. Studying the transcriptional control of pancreatic islet cells has important implications for understanding the mechanisms that control their normal development, as well as the pathogenesis of metabolic diseases such as diabetes. Histones represent the main protein components of the chromatin and undergo diverse covalent modifications that are very important for gene regulation. Here we describe the isolation of pancreatic islets from rodents and subsequently outline the methods used to immunoprecipitate and analyze the native chromatin obtained from these cells.

Tags
Antibody

Share this article

Published
January, 2020

Source

Products used in this publication

  • Antibody ChIP-seq grade icon
    C15410196
    H3K27ac polyclonal antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    C15410195
    H3K27me3 polyclonal antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    C15410194
    H3K4me1 polyclonal antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    C15410003-50
    H3K4me3 polyclonal antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    C15410193
    H3K9me3 polyclonal antibody - ChIP-seq Grade
  • default alt
    C03010020-220
    DiaMag protein A-coated magnetic beads (ChIP-se...

Events

  • EpiChrom
    Umea Sweden
    Feb 27-Feb 28, 2020
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics