Diagenode

DiaMag protein A-coated magnetic beads (ChIP-seq grade)

Catalog Number
Format
Price
C03010020-220
(kch-802-220)
220 µl
$110.00
Other format

The protein A-coated magnetic beads have been extensively validated in chromatin immunoprecipitation assay (ChIP). These beads are intended for isolation of immune complexes (chromatin and specific antibody) in ChIP experiments performed. The beads are suitable for immunoprecipitation of rabbit polyclonal Abs, mouse IgG2a, IgG2b and IgA, guinea pig IgG, dog IgG, pig IgG. The beads should be washed before use.

  • Characteristics

    Format
    Supplied as a suspension in PBS (pH 7.4), with 0.1% Tween-20 and 0.02% sodium azide.

    Storage and stability
    Store at 4°C. Do not freeze. Keep the beads in liquid suspension during storage as drying will result in reduced performance.

    Precautions
    This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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    Interleukin-22 protects intestinal stem cells against genotoxic stress.
    Gronke K, Hernández PP, Zimmermann J, Klose CSN, Kofoed-Branzk M, Guendel F, Witkowski M, Tizian C, Amann L, Schumacher F, Glatt H, Triantafyllopoulou A, Diefenbach A
    Environmental genotoxic factors pose a challenge to the genomic integrity of epithelial cells at barrier surfaces that separate host organisms from the environment. They can induce mutations that, if they occur in epithelial stem cells, contribute to malignant transformation and cancer development. Genome integrity ...

    H3K4/K9 acetylation and Lr28-mediated expression of six leaf rust responsive genes in wheat (Triticum aestivum).
    Sharma C, Kumar S, Saripalli G, Jain N, Raghuvanshi S, Sharma JB, Prabhu KV, Sharma PK, Balyan HS, Gupta PK
    Development of leaf rust-resistant cultivars is a priority during wheat breeding, since leaf rust causes major losses in yield. Resistance against leaf rust due to Lr genes is partly controlled by epigenetic modifications including histone acetylation that is known to respond to biotic/abiotic stresses. In the prese...

    The histone demethylase Jarid1b mediates angiotensin II-induced endothelial dysfunction by controlling the 3'UTR of soluble epoxide hydrolase.
    Vasconez AE, Janetzko P, Oo JA, Pflüger-Müller B, Ratiu C, Gu L, Helin K, Geisslinger G, Fleming I, Schröder K, Fork C, Brandes RP, Leisegang MS
    AIM: The histone demethylase Jarid1b limits gene expression by removing the active methyl mark from histone3 lysine4 at gene promoter regions. A vascular function of Jarid1b is unknown, but a vasoprotective function to inflammatory and hypertrophic stimuli, like angiotensin II (AngII) could be inferred. This hypothe...

    27nt-RNAs guide histone variant deposition via 'RNA-induced DNA replication interference' and thus transmit parental genome partitioning in Stylonychia.
    Postberg J, Jönsson F, Weil PP, Bulic A, Juranek SA, Lipps HJ
    BACKGROUND: During sexual reproduction in the unicellular ciliate Stylonychia somatic macronuclei differentiate from germline micronuclei. Thereby, programmed sequence reduction takes place, leading to the elimination of > 95% of germline sequences, which priorly adopt heterochromatin structure via ...

    Metabolic Induction of Trained Immunity through the Mevalonate Pathway.
    Bekkering S, Arts RJW, Novakovic B, Kourtzelis I, van der Heijden CDCC, Li Y, Popa CD, Ter Horst R, van Tuijl J, Netea-Maier RT, van de Veerdonk FL, Chavakis T, Joosten LAB, van der Meer JWM, Stunnenberg H, Riksen NP, Netea MG
    Innate immune cells can develop long-term memory after stimulation by microbial products during infections or vaccinations. Here, we report that metabolic signals can induce trained immunity. Pharmacological and genetic experiments reveal that activation of the cholesterol synthesis pathway, but not the synthesis of...

    Genomic Characterization of Metformin Hepatic Response
    Luizon M.R. et al.
    Metformin is used as a first-line therapy for type 2 diabetes (T2D) and prescribed for numerous other diseases. However, its mechanism of action in the liver has yet to be characterized in a systematic manner. To comprehensively identify genes and regulatory elements associated with metformin treatment, we carried o...

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