>>>   Click for Diagenode’s approach to COVID-19

A novel microscopy-based high-throughput screening method to identify proteins that regulate global histone modification levels.

Baas R, Lelieveld D, van Teeffelen H, Lijnzaad P, Castelijns B, van Schaik FM, Vermeulen M, Egan DA, Timmers HT, de Graaf P

Posttranslational modifications of histones play an important role in the regulation of gene expression and chromatin structure in eukaryotes. The balance between chromatin factors depositing (writers) and removing (erasers) histone marks regulates the steady-state levels of chromatin modifications. Here we describe a novel microscopy-based screening method to identify proteins that regulate histone modification levels in a high-throughput fashion. We named our method CROSS, for Chromatin Regulation Ontology SiRNA Screening. CROSS is based on an siRNA library targeting the expression of 529 proteins involved in chromatin regulation. As a proof of principle, we used CROSS to identify chromatin factors involved in histone H3 methylation on either lysine-4 or lysine-27. Furthermore, we show that CROSS can be used to identify chromatin factors that affect growth in cancer cell lines. Taken together, CROSS is a powerful method to identify the writers and erasers of novel and known chromatin marks and facilitates the identification of drugs targeting epigenetic modifications.


Share this article

February, 2014


Products used in this publication

  • Antibody ChIP-seq grade icon
    H3K27ac Antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    H3K4me1 Antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    H3K4me2 Antibody - ChIP-seq Grade
  • Antibody ChIP icon
    H3K27me1 polyclonal antibody
  • Antibody ChIP-seq grade icon
    H3K27me3 Antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    H3K4me3 Antibody - ChIP-seq Grade


 See all events


 See all news

The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics