Publications

Explore Diagenode’s publication database. Learn how our products are used in diverse fields of research.

1524 publications found×

Molecular analysis of enhancer RNAs and chromatin modifications in the region of their synthesis in Drosophila cells possessing genetic constructs.
Published: February, 2012

Abstract:
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Antibody

Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression.
Published: January, 2012

Abstract: ABSTRACT: BACKGROUND: DNA cytosine methylation is an epigenetic modification that has been implicated in many biological processes. However, large-scale epigenomic studies have been applied to very few plant species, and variability in methylatio...
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DNA shearing Bioruptor Antibody

Growth Arrest and DNA-Damage-Inducible, Beta (GADD45b)-Mediated DNA Demethylation in Major Psychosis.
Published: January, 2012

Abstract: Aberrant neocortical DNA methylation has been suggested to be a pathophysiological contributor to psychotic disorders. Recently, a growth arrest and DNA-damage-inducible, beta (GADD45b) protein-coordinated DNA demethylation pathway, utilizing cyti...
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Antibody 5mC (C15200081)

Novel transcriptional targets of the SRY-HMG box transcription factor SOX4 link its expression to the development of small cell lung cancer.
Published: January, 2012

Abstract: The HMG box transcription factor SOX4 involved in neuronal development is amplified and overexpressed in a subset of lung cancers, suggesting that it may be a driver oncogene. In this study, we sought to develop this hypothesis including by defini...
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Antibody

High-Mobility Group A1 Protein: A New Coregulator of Peroxisome Proliferator-Activated Receptor-γ-Mediated Transrepression in the Vasculature.
Published: December, 2011

Abstract: Rationale:The nuclear receptor peroxisome proliferator-activated receptor-γ (PPARγ) is an important regulator of gene transcription in vascular cells and mediates the vascular protection observed with antidiabetic glitazones.Objective:To determine...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody LowCell ChIP kit

Prepatterning of developmental gene expression by modified histones before zygotic genome activation.
Published: December, 2011

Abstract: A hallmark of anamniote vertebrate development is a window of embryonic transcription-independent cell divisions before onset of zygotic genome activation (ZGA). Chromatin determinants of ZGA are unexplored; however, marking of developmental genes...
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Antibody H3K4me3 (C15410003) H3K27me3 (C15410069)

Genome-wide profiling of LXR, RXR and PPARα in mouse liver reveals extensive sharing of binding sites.
Published: December, 2011

Abstract: The liver X receptors (LXRs) are nuclear receptors that form permissive heterodimers with retinoid X receptor (RXR) and are important regulators of lipid metabolism in the liver. We have recently shown that RXR agonist-induced hypertriglyceridemia...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

Tracheobronchial transplantation with a stem-cell-seeded bioartificial nanocomposite: a proof-of-concept study.
Published: December, 2011

Abstract: BACKGROUND: Tracheal tumours can be surgically resected but most are an inoperable size at the time of diagnosis; therefore, new therapeutic options are needed. We report the clinical transplantation of the tracheobronchial airway with a stem-cel...
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Antibody LowCell ChIP kit

Mechanisms and functions of Tet protein-mediated 5-methylcytosine oxidation.
Published: December, 2011

Abstract: Ten-eleven translocation 1-3 (Tet1-3) proteins have recently been discovered in mammalian cells to be members of a family of DNA hydroxylases that possess enzymatic activity toward the methyl mark on the 5-position of cytosine (5-methylcytosine [5...
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Antibody

Distinct Epigenomic Features in End-Stage Failing Human Hearts
Published: November, 2011

Abstract: Background—The epigenome refers to marks on the genome, including DNA methylation and histone modifications, that regulate the expression of underlying genes. A consistent profile of gene expression changes in end-stage cardiomyopathy led us to hy...
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DNA shearing Bioruptor Chromatin Shearing ChIP-seq Antibody

Generation and characterization of rat and mouse monoclonal antibodies specific for MeCP2 and their use in X-inactivation studies.
Published: November, 2011

Abstract: Methyl CpG binding protein 2 (MeCP2) binds DNA, and has a preference for methylated CpGs and, hence, in cells, it accumulates in heterochromatin. Even though it is expressed ubiquitously MeCP2 is particularly important during neuronal maturation. ...
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Bioruptor Chromatin Shearing ChIP-qPCR

Pre-B cell to macrophage transdifferentiation without significant promoter DNA methylation changes.
Published: November, 2011

Abstract: Transcription factor-induced lineage reprogramming or transdifferentiation experiments are essential for understanding the plasticity of differentiated cells. These experiments helped to define the specific role of transcription factors in conferr...
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Antibody MethylCap

Bivalent histone modifications in stem cells poise miRNA loci for CpG island hypermethylation in human cancer.
Published: November, 2011

Abstract: It has been proposed that the existence of stem cell epigenetic patterns confer a greater likelihood of CpG island hypermethylation on tumor suppressor-coding genes in cancer. The suggested mechanism is based on the Polycomb-mediated methylation o...
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Antibody LowCell ChIP kit

PcG complexes set the stage for epigenetic inheritance of gene silencing in early S phase before replication.
Published: November, 2011

Abstract: Polycomb group (PcG) proteins are part of a conserved cell memory system that conveys epigenetic inheritance of silenced transcriptional states through cell division. Despite the considerable amount of information about PcG mechanisms controlling ...
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Antibody

Pharmacodynamics of curcumin as DNA hypomethylation agent in restoring the expression of Nrf2 via promoter CpGs demethylation.
Published: November, 2011

Abstract: Prostate cancer (PCa) is one of the most deadly malignancies among men in the United States. Although localized prostate cancer can be effectively treated via surgery or radiation, metastatic disease is usually lethal. Recent evidence suggests tha...
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DNA shearing Bioruptor

CTCF-promoted RNA polymerase II pausing links DNA methylation to splicing.
Published: October, 2011

Abstract: Alternative splicing of pre-messenger RNA is a key feature of transcriptome expansion in eukaryotic cells, yet its regulation is poorly understood. Spliceosome assembly occurs co-transcriptionally, raising the possibility that DNA structure may di...
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DNA shearing Bioruptor Chromatin Shearing ChIP-qPCR Antibody

The human histone H3 complement anno 2011.
Published: October, 2011

Abstract: Histones are highly basic, relatively small proteins that complex with DNA to form higher order structures that underlie chromosome topology. Of the four core histones H2A, H2B, H3 and H4, it is H3 that is most heavily modified at the post-transla...
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Antibody

DNA methylation profiles of human active and inactive X chromosomes.
Published: October, 2011

Abstract: X-chromosome inactivation (XCI) is a dosage compensation mechanism that silences the majority of genes on one X chromosome in each female cell. To characterize epigenetic changes that accompany this process, we measured DNA methylation levels in 4...
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Antibody

Histone tail acetylation in brain occurs in an unpredictable fashion after death.
Published: September, 2011

Abstract: Histone acetylation plays a role in the regulation of gene transcription. Yet it is not known whether post-mortem brain tissue is suitable for the analysis of histone acetylation. To examine this question, nucleosomes were isolated from frontal co...
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Antibody LowCell ChIP kit

Reciprocal repression between Sox3 and snail transcription factors defines embryonic territories at gastrulation.
Published: September, 2011

Abstract: In developing amniote embryos, the first epithelial-to-mesenchymal transition (EMT) occurs at gastrulation, when a subset of epiblast cells moves to the primitive streak and undergoes EMT to internalize and generate the mesoderm and the endoderm. ...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

Glutathione-S-transferase pi 1(GSTP1) gene silencing in prostate cancer cells is reversed by the histone deacetylase inhibitor depsipeptide.
Published: September, 2011

Abstract: Gene silencing by epigenetic mechanisms is frequent in prostate cancer (PCA). The link between DNA hypermethylation and histone modifications is not completely understood. We chose the GSTP1 gene which is silenced by hypermethylation to analyze th...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

Silencing of Kruppel-like factor 2 by the histone methyltransferase EZH2 in human cancer.
Published: September, 2011

Abstract: The Kruppel-like factor (KLF) proteins are multitasked transcriptional regulators with an expanding tumor suppressor function. KLF2 is one of the prominent members of the family because of its diminished expression in malignancies and its growth-i...
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Antibody H3K27me3 (C15410069)

Disruption of the histone acetyltransferase MYST4 leads to a Noonan syndrome-like phenotype and hyperactivated MAPK signaling in humans and mice.
Published: September, 2011

Abstract: Epigenetic regulation of gene expression, through covalent modification of histones, is a key process controlling growth and development. Accordingly, the transcription factors regulating these processes are important targets of genetic diseases. ...
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Bioruptor Chromatin Shearing Antibody LowCell ChIP kit

Improved quantitative and qualitative production of single-domain intrabodies mediated by the co-expression of Erv1p sulfhydryl oxidase.
Published: September, 2011

Abstract: Camelidae single domain antibodies (VHHs) have structural and binding features that render them suitable alternatives to conventional IgG antibodies. VHHs are usually easier to produce as recombinant proteins than other antibody fragments. However...
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Bioruptor Cell Lysis

Lineage-specific distribution of high levels of genomic 5-hydroxymethylcytosine in mammalian development.
Published: September, 2011

Abstract: Methylation of cytosine is a DNA modification associated with gene repression. Recently, a novel cytosine modification, 5-hydroxymethylcytosine (5-hmC) has been discovered. Here we examine 5-hmC distribution during mammalian development and in cel...
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Antibody

Effects of genome architecture and epigenetic factors on susceptibility of promoter CpG islands to aberrant DNA methylation induction.
Published: September, 2011

Abstract: Aberrant DNA methylation is induced at specific promoter CpG islands (CGIs) in contrast with mutations. The specificity is influenced by genome architecture and epigenetic factors, but their relationship is still unknown. In this study, we isolate...
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Antibody

Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes.
Published: September, 2011

Abstract: Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component...
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Antibody

Selective autophagy regulates insertional mutagenesis by the Ty1 retrotransposon in Saccharomyces cerevisiae.
Published: August, 2011

Abstract: Macroautophagy (autophagy) is a bulk degradation system for cytoplasmic components and is ubiquitously found in eukaryotic cells. Autophagy is induced under starvation conditions and plays a cytoprotective role by degrading unwanted cytoplasmic ma...
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Antibody

The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters.
Published: August, 2011

Abstract: The human tumour antigen PRAME (preferentially expressed antigen of melanoma) is frequently overexpressed in tumours. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in...
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Bioruptor Chromatin Shearing ChIP-seq Antibody

IL-20 is epigenetically regulated in NSCLC and down regulates the expression of VEGF.
Published: August, 2011

Abstract: BACKGROUND: IL-20 is a pleiotrophic member of the IL-10 family and plays a role in skin biology and the development of haematopoietic cells. Recently, IL-20 has been demonstrated to have potential anti-angiogenic effects in non-small cell lung can...
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Antibody H3K4me3 (C15410003)

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