Publications

Explore Diagenode’s publication database. Learn how our products are used in diverse fields of research.

1474 publications found×

Limitations and possibilities of low cell number ChIP-seq.
Published: November, 2012

Abstract: ABSTRACT: BACKGROUND: Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting materi...
Read more »

Tags
Bioruptor Chromatin Shearing ChIP-seq Antibody H3K4me3 (C15410003)

Protein Group Modification and Synergy in the SUMO Pathway as Exemplified in DNA Repair.
Published: November, 2012

Abstract: Protein modification by SUMO affects a wide range of protein substrates. Surprisingly, although SUMO pathway mutants display strong phenotypes, the function of individual SUMO modifications is often enigmatic, and SUMOylation-defective mutants com...
Read more »

Tags
Bioruptor Chromatin Shearing ChIP-qPCR Antibody H3K4me3 (C15410003)

Inhibition of Tumor Promotion by Parthenolide: Epigenetic Modulation of p21.
Published: November, 2012

Abstract: The promotion stage in the multistep process of epidermal tumorigenesis is NF-кB-dependent, epigenetically regulated, and reversible, thus, a suitable target for chemoprevention. We investigated whether the NF-кB inhibitor, parthenolide, currently...
Read more »

Tags
Antibody

Thalamus-derived molecules promote survival and dendritic growth of developing cortical neurons.
Published: October, 2012

Abstract: The mammalian neocortex is composed of various types of neurons that reflect its laminar and area structures. It has been suggested that not only intrinsic but also afferent-derived extrinsic factors are involved in neuronal differentiation during...
Read more »

Tags
Antibody

IL-23 is pro-proliferative, epigenetically regulated and modulated by chemotherapy in non-small cell lung cancer.
Published: October, 2012

Abstract: BACKGROUND: IL-23 is a member of the IL-6 super-family and plays key roles in cancer. Very little is currently known about the role of IL-23 in non-small cell lung cancer (NSCLC). METHODS: RT-PCR and chromatin immunopreciptiation (ChIP) were used ...
Read more »

Tags
Antibody H3K4me3 (C15410003)

High-resolution detection of DNA binding sites of the global transcriptional regulator GlxR in Corynebacterium glutamicum.
Published: October, 2012

Abstract: The transcriptional regulator GlxR has been characterised as a global hub within the gene-regulatory network of Corynebacterium glutamicum. Chromatin immunoprecipitation with a specific anti-GlxR antibody and subsequent high-throughput sequencing ...
Read more »

Tags
Bioruptor Chromatin Shearing ChIP-seq

Periodontitis in established rheumatoid arthritis patients: a cross-sectional clinical, microbiological and serological study.
Published: October, 2012

Abstract: ABSTRACT: INTRODUCTION: The association between rheumatoid arthritis (RA) and periodontitis is suggested to be linked to the periodontal pathogen Porphyromonas gingivalis. Colonization of P. gingivalis in the oral cavity of RA patients has been sc...
Read more »

Tags
Bioruptor Cell Lysis Enzymatic Assay

New partners in regulation of gene expression: the enhancer of trithorax and polycomb corto interacts with methylated ribosomal protein l12 via its chromodomain.
Published: October, 2012

Abstract: Chromodomains are found in many regulators of chromatin structure, and most of them recognize methylated lysines on histones. Here, we investigate the role of the Drosophila melanogaster protein Corto's chromodomain. The Enhancer of Trithorax and ...
Read more »

Tags
Bioruptor Chromatin Shearing ChIP-qPCR Antibody H3K4me3 (C15410003) H3K27me3 (C15410069) IPure kit

Non-genotoxic carcinogen exposure induces defined changes in the 5-hydroxymethylome.
Published: October, 2012

Abstract: ABSTRACT: BACKGROUND: Induction and promotion of liver cancer by exposure to non-genotoxic carcinogens coincides with epigenetic perturbations, including specific changes in DNA methylation. Here we investigate the genome-wide dynamics of 5-hydro...
Read more »

Tags
DNA shearing Bioruptor Antibody

Human β cell transcriptome analysis uncovers lncRNAs that are tissue-specific, dynamically regulated, and abnormally expressed in type 2 diabetes.
Published: October, 2012

Abstract: A significant portion of the genome is transcribed as long noncoding RNAs (lncRNAs), several of which are known to control gene expression. The repertoire and regulation of lncRNAs in disease-relevant tissues, however, has not been systematically ...
Read more »

Tags
Antibody

Features of the Arabidopsis recombination landscape resulting from the combined loss of sequence variation and DNA methylation.
Published: October, 2012

Abstract: The rate of meiotic crossing over (CO) varies considerably along chromosomes, leading to marked distortions between physical and genetic distances. The causes underlying this variation are being unraveled, and DNA sequence and chromatin states hav...
Read more »

Tags
DNA shearing Bioruptor Antibody

Epigenetic transgenerational inheritance of vinclozolin induced mouse adult onset disease and associated sperm epigenome biomarkers.
Published: October, 2012

Abstract: The endocrine disruptor vinclozolin has previously been shown to promote epigenetic transgenerational inheritance of adult onset disease in the rat. The current study was designed to investigate the transgenerational actions of vinclozolin on the ...
Read more »

Tags
Antibody

ChIP-exo Method for Identifying Genomic Location of DNA-Binding Proteins with Near-Single-Nucleotide Accuracy.
Published: October, 2012

Abstract: This unit describes the ChIP-exo methodology, which combines chromatin immunoprecipitation (ChIP) with lambda exonuclease digestion followed by high-throughput sequencing. ChIP-exo allows identification of a nearly complete set of the binding loca...
Read more »

Tags
Bioruptor Chromatin Shearing ChIP-seq

Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation.
Published: September, 2012

Abstract: A fundamental challenge in mammalian biology has been the elucidation of mechanisms linking DNA methylation and histone post-translational modifications. Human UHRF1 (ubiquitin-like PHD and RING finger domain-containing 1) has multiple domains tha...
Read more »

Tags
Antibody 5mC (C15200081)

Bacille Calmette-Guerin induces NOD2-dependent nonspecific protection from reinfection via epigenetic reprogramming of monocytes.
Published: September, 2012

Abstract: Adaptive features of innate immunity, recently described as "trained immunity," have been documented in plants, invertebrate animals, and mice, but not yet in humans. Here we show that bacille Calmette-Guérin (BCG) vaccination in healthy volunteer...
Read more »

Tags
Antibody

p53-Independent regulation of p21Waf1/Cip1 expression and senescence by PRMT6.
Published: September, 2012

Abstract: p21 is a potent cyclin-dependent kinase inhibitor that plays a role in promoting G1 cell cycle arrest and cellular senescence. Consistent with this role, p21 is a downstream target of several tumour suppressors and oncogenes, and it is downregulat...
Read more »

Tags
Antibody

ERG and FLI1 binding sites demarcate targets for aberrant epigenetic regulation by AML1-ETO in acute myeloid leukemia.
Published: September, 2012

Abstract: ERG and FLI1 are closely related members of the ETS family of transcription factors and have been identified as essential factors for the function and maintenance of normal hematopoietic stem cells. Here, genome-wide analysis revealed that both ER...
Read more »

Tags
IP-Star Antibody

H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation
Published: September, 2012

Abstract: The Caenorhabditis elegans dosage compensation complex (DCC) equalizes X-chromosome gene dosage between XO males and XX hermaphrodites by two-fold repression of X-linked gene expression in hermaphrodites. The DCC localizes to the X chromosomes in ...
Read more »

Tags
Antibody

Tet2 Facilitates the Derepression of Myeloid Target Genes during CEBPα-Induced Transdifferentiation of Pre-B Cells.
Published: September, 2012

Abstract: The methylcytosine hydroxylase Tet2 has been implicated in hematopoietic differentiation and the formation of myeloid malignancies when mutated. An ideal system to study the role of Tet2 in myelopoeisis is CEBPα-induced transdifferentiation of pre...
Read more »

Tags
DNA shearing Bioruptor Antibody

Pesticide and Insect Repellent Mixture (Permethrin and DEET) Induces Epigenetic Transgenerational Inheritance of Disease and Sperm Epimutations.
Published: September, 2012

Abstract: Environmental compounds are known to promote epigenetic transgenerational inheritance of disease. The current study was designed to determine if a "pesticide mixture" (pesticide permethrin and insect repellent N,N-Diethyl-meta-toluamide, DEET) pro...
Read more »

Tags
Antibody

Multigenerational epigenetic adaptation of the hepatic wound-healing response.
Published: September, 2012

Abstract: We investigated whether ancestral liver damage leads to heritable reprogramming of hepatic wound healing in male rats. We found that a history of liver damage corresponds with transmission of an epigenetic suppressive adaptation of the fibrogenic ...
Read more »

Tags
Bioruptor Chromatin Shearing ChIP-qPCR Antibody H3K27me3 (C15410069)

Chromatin accessibility, p300 and histone acetylation define PML-RARα and AML1-ETO binding sites in acute myeloid leukemia.
Published: August, 2012

Abstract: Chromatin accessibility plays a key role in regulating cell type specific gene expression during hematopoiesis, but has also been suggested to be aberrantly regulated during leukemogenesis. To understand the leukemogenic chromatin signature we ana...
Read more »

Tags
Antibody

Candida albicans Infection Affords Protection against Reinfection via Functional Reprogramming of Monocytes.
Published: August, 2012

Abstract: Immunological memory in vertebrates is often exclusively attributed to T and B cell function. Recently it was proposed that the enhanced and sustained innate immune responses following initial infectious exposure may also afford protection against...
Read more »

Tags
Antibody

Histone acetylation and DNA demethylation of T-cells result in an anaplastic large cell lymphoma-like phenotype.
Published: August, 2012

Abstract: Background. A characteristic feature of anaplastic large cell lymphoma is the significant repression of the T-cell expression program despite its T-cell origin. The reasons for this down-regulation of T-cell phenotype are still unknown. Design and...
Read more »

Tags
Antibody 5mC (C15200081)

The Human EKC/KEOPS Complex Is Recruited to Cullin2 Ubiquitin Ligases by the Human Tumour Antigen PRAME.
Published: August, 2012

Abstract: The human tumour antigen PRAME (preferentially expressed antigen in melanoma) is frequently overexpressed during oncogenesis, and high PRAME levels are associated with poor clinical outcome in a variety of cancers. However, the molecular pathways ...
Read more »

Tags
Antibody

A Sharp Cadherin-6 Gene Expression Boundary in the Developing Mouse Cortical Plate Demarcates the Future Functional Areal Border.
Published: August, 2012

Abstract: The mammalian cerebral cortex can be tangentially subdivided into tens of functional areas with distinct cyto-architectures and neural circuitries; however, it remains elusive how these areal borders are genetically elaborated during development. ...
Read more »

Tags
Antibody

Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription.
Published: August, 2012

Abstract: Nucleolin is a multi-functional nucleolar protein that is required for ribosomal RNA gene (rRNA) transcription in vivo, but the mechanism by which nucleolin modulates RNA polymerase I (RNAPI) transcription is not well understood. Nucleolin depleti...
Read more »

Tags
Antibody

Chromatin Immunoprecipitation Analysis of Xenopus Embryos
Published: August, 2012

Abstract: Chromatin immunoprecipitation (ChIP) is a powerful technique to study epigenetic regulation and transcription factor binding events in the nucleus. It is based on immune-affinity capture of epitopes that have been cross-linked to genomic DNA in vi...
Read more »

Tags
Bioruptor Chromatin Shearing ChIP-qPCR Antibody

The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology and the metabolome of fibroblasts
Published: July, 2012

Abstract: Fbxl10 (Jhdm1b/Kdm2b) is a conserved and ubiquitously expressed member of the JHDM (JmjC-domain-containing histone demethy-lase) family. Fbxl10 was implicated in the demethylation of H3K4me3 or H3K36me2 thereby removing active chromatin marks and ...
Read more »

Tags
Antibody H3K27me3 (C15410069)

A comparison of transcriptome and epigenetic status between closely related species in the genus Arabidopsis.
Published: July, 2012

Abstract: Difference in the level of expression of genes is one of the factors contributing to plant phenotype. As well as being under genetic control, gene expression is regulated by epigenetic processes such as DNA methylation and histone modifications. W...
Read more »

Tags
Antibody

Publications

Events