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Polyclonal antibody raised in rabbit against human ZBTB38 (zinc finger and BTB domain containing 38), using three KLH-conjugated synthetic peptides, two containing a sequence from the central part and one containing a sequence from the C-terminal part of the protein.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ZBTB38 (Cat. No. CS-123-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:26,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against ZBTB38 Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against ZBTB38 (Cat. No. CS-123-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 138 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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