UHRF1 Antibody

Catalog Number
100 μl
  Bulk order

Polyclonal antibody raised in rabbit against UHRF1 (ubiquitin-like with PHD and ring finger domains 1), using a recombinant protein.

Concentration1.35 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 5
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 3

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data

    UHRF1 Antibody ChIP Grade

    Figure 1. UHRF1 ChIP results
    ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.

    UHRF1 Antibody validated in Immunoprecipitation

    Figure 2. IP results
    UHRF1 antibody immunoprecipitates UHRF1 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 μg of UHRF1 antibody (C15410258) 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by western blot with the UHRF1 antibody (C15410258) diluted 1:1,000.

    UHRF1 Antibody validated in Western Blot

    Figure 3. Western blot
    Sample: 30 μg of HCT116 whole cell lysate 7.5% SDS PAGE UHRF1 diluted 1:1,000

    UHRF1 Antibody validated in immunohistochemical

    Figure 4. IHC
    UHRF1 antibody detects UHRF1 protein on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. UHRF1 antibody (C15410258) dilution: 1:500.

    UHRF1 Antibody validated in immunofluorescence

    Figure 5. IFA
    Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using UHRF1 antibody (Cat. No. C15410258) (Green) at a 1:500 dilution. Red: Alpha-tubulin filaments.

  •  Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunohistochemistry Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet UHRF1 C15410258 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: UHRF1 Antibody (Diagenode Cat# C15410258-100 Lot# 44797). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Novel role of UHRF1 in the epigenetic repression of the latent HIV-1.
    Verdikt R. et al.
    BACKGROUND: The multiplicity, heterogeneity, and dynamic nature of human immunodeficiency virus type-1 (HIV-1) latency mechanisms are reflected in the current lack of functional cure for HIV-1. Accordingly, all classes of latency-reversing agents (LRAs) have been reported to present variable ex vivo potencies. Here,...

    DNA methylation enzymes and PRC1 restrict B-cell Epstein-Barr virus oncoprotein expression.
    Guo R, Zhang Y, Teng M, Jiang C, Schineller M, Zhao B, Doench JG, O'Reilly RJ, Cesarman E, Giulino-Roth L, Gewurz BE
    To accomplish the remarkable task of lifelong infection, the Epstein-Barr virus (EBV) switches between four viral genome latency and lytic programmes to navigate the B-cell compartment and evade immune responses. The transforming programme, consisting of highly immunogenic EBV nuclear antigen (EBNA) and latent membr...

  •  Related products


  • EpiPlant 2024
    Clermont-Ferrand, France
    Jul 10-Jul 12, 2024
 See all events


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy