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Figure 1. UHRF1 ChIP results ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.
Figure 2. IP results UHRF1 antibody immunoprecipitates UHRF1 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 μg of UHRF1 antibody (C15410258) 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by western blot with the UHRF1 antibody (C15410258) diluted 1:1,000.
Figure 3. Western blot Sample: 30 μg of HCT116 whole cell lysate 7.5% SDS PAGE UHRF1 diluted 1:1,000
Figure 4. IHC UHRF1 antibody detects UHRF1 protein on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. UHRF1 antibody (C15410258) dilution: 1:500.
Figure 5. IFA Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using UHRF1 antibody (Cat. No. C15410258) (Green) at a 1:500 dilution. Red: Alpha-tubulin filaments.
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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