SMAD2 polyclonal antibody - Classic

Figure 1. SMAD2 antibody Western blot results
Western blot using the Diagenode antibody against SMAD2 to detect over-expressed SMAD2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of SMAD2 in lysates of COS transfected with SMAD2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGFß. Low levels of staining in control lanes correspond to detection of endogenous SMAD2. The membrane was probed with the primary antibody at a 1:2500 dilution.