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SMAD2 polyclonal antibody - Classic

Catalog Number
100 μg
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Polyclonal antibody raised in rabbit against SMAD2, using a KLH-conjugated synthetic peptide.

Concentration0.55 μg/μl
Species reactivityHuman, mouse, rat
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100,000
Western Blotting 1:500 - 1:3,000 Fig 1
  • Validation Data

    Western blot results

    Figure 1. SMAD2 antibody Western blot results
    Western blot using the Diagenode antibody against SMAD2 to detect over-expressed SMAD2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of SMAD2 in lysates of COS transfected with SMAD2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGFß. Low levels of staining in control lanes correspond to detection of endogenous SMAD2. The membrane was probed with the primary antibody at a 1:2500 dilution.

  •  Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    Datasheet SMAD2 C15410273 DATASHEET
    Datasheet description
  •  Publications

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