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SMAD2 polyclonal antibody

Catalog Number
Format
Price
C15410273
100 μg
$355.00
  Bulk order



Polyclonal antibody raised in rabbit against SMAD2, using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.55 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100,000
Western Blotting 1:500 - 1:3,000 Fig 1
  • Validation Data

    Western blot results

    Figure 1. SMAD2 antibody Western blot results
    Western blot using the Diagenode antibody against SMAD2 to detect over-expressed SMAD2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of SMAD2 in lysates of COS transfected with SMAD2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGFß. Low levels of staining in control lanes correspond to detection of endogenous SMAD2. The membrane was probed with the primary antibody at a 1:2500 dilution.

  •  Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    Datasheet SMAD2 C15410273 DATASHEET
    Datasheet description
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD2 polyclonal antibody (Diagenode Cat# C15410273 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The TGF-β profibrotic cascade targets ecto-5'-nucleotidase gene in proximal tubule epithelial cells and is a traceable marker of progressive diabetic kidney disease.
    Cappelli C, Tellez A, Jara C, Alarcón S, Torres A, Mendoza P, Podestá L, Flores C, Quezada C, Oyarzún C, Martín RS
    Progressive diabetic nephropathy (DN) and loss of renal function correlate with kidney fibrosis. Crosstalk between TGF-β and adenosinergic signaling contributes to the phenotypic transition of cells and to renal fibrosis in DN models. We evaluated the role of TGF-β on NT5E gene expression coding for the ec...

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