p300 Antibody (sample size)

Catalog Number
10 µg
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Alternative names: EP300, KAT3B, RSTS2

Monoclonal antibody raised in mouse against human p300 (E1A Binding Protein P300) by DNA immunization in which the C-terminal part of the protein was cloned and expressed.

Concentration1.8 µg/µl
Species reactivityHuman. Other species: not tested.
PurityProtein A purified monoclonal antibody
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2-5 μg/ChIP Fig 1, 2
Western Blotting Not Recommended

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation data

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against p300

    ChIP was performed using HeLa cells, the Diagenode monoclonal antibody against p300 (cat. No. C15200211) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.p300 Antibody ChIP-seq Grade

    B.p300 Antibody for ChIP-seq

    C.p300 Antibody for ChIP-seq assay

    D.p300 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against p300

    ChIP was performed with 5 µg of the Diagenode antibody against p300 (cat. No. C15200211) on sheared chromatin from 4 million HeLa cells as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 mb region of chromosome 5 (figure 2A and B) and in two regions surrounding the IRS2 and ANKRD32 (SLF1) positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.

  • Target Description

    p300 (UniProt/Swiss-Prot entry Q09472) is a histone acetyltransferase that regulates transcription via chromatin remodelling. As such it is important for cell proliferation and differentiation. p300 is able to acetylate all four core histones in nucleosomes. Acetylation of histones is associated with transcriptional activation. p300 also acetylates non-histone proteins such as HDAC1 leading to its inactivation and modulation of transcription. It has also been identified as a co-activator of HIF1A (hypoxiainducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in the p300 gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

  •  Applications
  •  Documents
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Datasheet p300 C15200211 DATASHEET
    Datasheet description
  •  Safety sheets
    p300 Antibody SDS GB en Download
    p300 Antibody SDS US en Download
    p300 Antibody SDS BE nl Download
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    p300 Antibody SDS JP ja Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: p300 Antibody (sample size) (Diagenode Cat# C15200211-10 Lot# 002). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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    Niche-Specific Reprogramming of Epigenetic Landscapes Drives Myeloid Cell Diversity in Nonalcoholic Steatohepatitis.
    Seidman JS, Troutman TD, Sakai M, Gola A, Spann NJ, Bennett H, Bruni CM, Ouyang Z, Li RZ, Sun X, Vu BT, Pasillas MP, Ego KM, Gosselin D, Link VM, Chong LW, Evans RM, Thompson BM, McDonald JG, Hosseini M, Witztum JL, Germain RN, Glass CK
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