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Polyclonal antibody raised in rabbit against human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the α and γ isoforms.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against HP1α, ß and γ ChIP assays were performed using NIH3T3 cells and 4 μg of the Diagenode antibody directed against HP1α, ß and γ (Cat. No. C15410071). QPCR was performed on the IP’d DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure 1 shows the relative enrichment as compared to a no antibody negative control ChIP.
Figure 2. Western blot analysis using the Diagenode antibody directed against HP1α, ß and γ Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human HP1α, ß and γ (Cat. No. C15410071) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1ß and HP1γ is indicated on the right.
Figure 3. Immunofluorescence using the Diagenode antibody directed against HP1α, ß and γ HeLa cells were stained with the Diagenode antibody against HP1α, ß and γ (Cat. No. C15410071) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α, ß and γ antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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