HP1?, ß and ? polyclonal antibody

Catalog Number
50 µg
  Bulk order

Alternative names: CBX5, 1, 3

Polyclonal antibody raised in rabbit against human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the α and γ isoforms.

Concentration2.0 µg/µl
Species reactivityHuman, mouse: positive. Other species: not tested
PurityProtein G purified polyclonal antibody
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide and 0.05% ProClin 300
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:500 Fig 2
  • Validation Data

    Western blot

    Figure 1. Western blot analysis using the Diagenode antibody directed against HP1α, ß and γ
    Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human HP1α, ß and γ (Cat. No. C15410071) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1ß and HP1γ is indicated on the right.


    Figure 2. Immunofluorescence using the Diagenode antibody directed against HP1α, ß and γ
    HeLa cells were stained with the Diagenode antibody against HP1α, ß and γ (Cat. No. C15410071) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α, ß and γ antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  •  実験手法
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  資料
    Datasheet hHP1 pAb-071-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    HP1 antibody SDS GB en Download
    HP1 antibody SDS US en Download
    HP1 antibody SDS BE nl Download
    HP1 antibody SDS BE fr Download
    HP1 antibody SDS FR fr Download
    HP1 antibody SDS ES es Download
    HP1 antibody SDS DE de Download
    HP1 antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HP1?, ß and ? polyclonal antibody (Diagenode Cat# C15410071 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Spatiotemporal control of estrogen-responsive transcription in ERα-positive breast cancer cells.
    P-Y Hsu, H-K Hsu, T-H Hsiao, Z Ye, E Wang, A L Profit, I Jatoi, Y Chen, N B Kirma, V X Jin, Z D Sharp and T H-M Huang
    Recruitment of transcription machinery to target promoters for aberrant gene expression has been well studied, but underlying control directed by distant-acting enhancers remains unclear in cancer development. Our previous study demonstrated that distant estrogen response elements (DEREs) located on chromosome 20q13...


  • ESHG 2024
    Berlin, Germany
    Jun 1-Jun 4, 2024
  • CLEPIC 2024
    Warsaw, Poland
    Jun 5-Jun 7, 2024
  • EACR 2024
    Rotterdam, Netherlands
    Jun 10-Jun 13, 2024
  • Chromatin meets South 2024
    Marseille, France
    Jun 13-Jun 14, 2024


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics