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Polyclonal antibody raised in rabbit against human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the α and γ isoforms.
Lot
001
Concentration
2.0 µg/µl
Species reactivity
Human, mouse: positive. Other species: not tested
Type
Polyclonal
Purity
Protein G purified polyclonal antibody
Host
Rabbit
Storage Conditions
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer
PBS containing 0.05% azide and 0.05% ProClin 300
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications
Suggested dilution
References
Western Blotting
1:1,000
Fig 1
Immunofluorescence
1:500
Fig 2
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.
Figure 1. Western blot analysis using the Diagenode antibody directed against HP1α, ß and γ Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human HP1α, ß and γ (Cat. No. C15410071) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1ß and HP1γ is indicated on the right.
Figure 2. Immunofluorescence using the Diagenode antibody directed against HP1α, ß and γ HeLa cells were stained with the Diagenode antibody against HP1α, ß and γ (Cat. No. C15410071) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α, ß and γ antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
Learn more about: Load... Read more
IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
Diagenode strongly recommends using this: HP1?, ß and ? polyclonal antibody (Diagenode Cat# C15410071 Lot# 001). Click here to copy to clipboard.
Using our products in your publication? Let us know!
Spatiotemporal control of estrogen-responsive transcription in ERα-positive breast cancer cells. P-Y Hsu, H-K Hsu, T-H Hsiao, Z Ye, E Wang, A L Profit, I Jatoi, Y Chen, N B Kirma, V X Jin, Z D Sharp and T H-M Huang Recruitment of transcription machinery to target promoters for aberrant gene expression has been well studied, but underlying control directed by distant-acting enhancers remains unclear in cancer development. Our previous study demonstrated that distant estrogen response elements (DEREs) located on chromosome 20q13...
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