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H3K18me1 Antibody - ChIP Grade

Catalog Number
Format
Price
C15410290
50 μg
$380.00
  Bulk order



Polyclonal antibody raised in rabbit against against histone H3, monomethylated at lysine 18 (H3K18me1), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.65 μg/μl
Species reactivityHuman, mouse, rat, chicken, C. elegans, Xenopus, Drosophila, plants
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP * 2 μg/ChIP Fig 1
Dot Blotting 1:1,000 Fig 2
Western Blotting 1:500 Fig 3
Immunofluorescence 1:50 Fig 4
IHC 1:100

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    H3K18me1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K18me1
    ChIP assays were performed using 2 μg of the Diagenode antibody against H3K18me1 (cat. No. C15410290) on sheared chromatin from 1 million cells. A no antibody negative IP control was also included. QPCR was performed with primers for the indicated genes or genomic regions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    H3K18me1 Antibody validation in Dot Blot

    Figure 2. Cross reactivity test of the Diagenode antibody directed against H3K18me1
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K18me1 (cat. No. C15410290) with peptides containing other modifications of H3K18 and the unmodified sequence. One hundred, 10 and 1 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    H3K18me1 Antibody validation in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against H3K18me1
    Western blot was performed on histone extracts (30 μg) from HeLa (lane 1) and NIH3T3 cells (lane 2) using the Diagenode antibody against H3K18me1 (cat. No. C15410290) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    H3K18me1 Antibody validation in Immunofluorescence

    Figure 4. Immunofluorescence using the Diagenode antibody directed against H3K18me1
    HeLa cells were stained with the Diagenode antibody against H3K18me1 (cat. No. C15410190) (green), with an anti-actin antibody (red) and with DAPI (blue). Cells were fixed with 0.5% formaldehyde. The cells were incubated with the H3K18me1 antibody diluted 1:50 in blocking solution for 1h at RT, followed by an anti-rabbit antibody conjugated to FITC. The lower right panel shows a merge of the three stainings.

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

  •  Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3K18me1 C15410290 DATASHEET
    Polyclonal antibody raised in rabbit against against histone H3, monomethylated at lysine 18 (H3K...
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  •  Publications

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