Figure 1. ChIP results obtained with the Diagenode antibody directed against H2AK119ub.
ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2AK119ub (cat. No. C15410002) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (cat. No. AB-Auto02-A100), using sheared chromatin from 1 million cells on the SX-8G IP-Star automated system. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and c-fos genes, used as negative controls, and for the inactive MYT1 and TSH2B genes, used as a positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).