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Figure 1. FUBP1 ChIP results ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.
Figure 2. FUBP1 IP results FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.
Figure 3. IHC Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.
Figure 4. Western blot Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% SDS PAGE FUBP1 antibody diluted 1:5,000
Figure 5. FUBP1 IF results FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
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WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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