Diagenode

FUBP1 polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15410233-100
100 μl
$345.00
  Bulk order

Polyclonal antibody raised in rabbit against FUBP1 (far upstream element-binding protein 1), using a recombinant protein.

Lot40912
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 5
IHC 1:100 - 1:1,000 Fig 3
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:100 - 1:1,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    FUBP1 ChIP results

    Figure 1. FUBP1 ChIP results
    ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.

    FUBP1 IP results

    Figure 2. FUBP1 IP results
    FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.

    IHC

    Figure 3. IHC
    Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.

    Western blot

    Figure 4. Western blot
    Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% SDS PAGE FUBP1 antibody diluted 1:5,000

    IF results

    Figure 5. FUBP1 IF results
    FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.

  •  実験手法
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet FUBP1 C15410233 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  出版物

    How to properly cite this product in your work

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