Diagenode

ELAVL2 polyclonal antibody

Catalog Number
Format
Price
C15410347
100 µg
$315.00
  Bulk order

Other names: HUB, HELN1, HEL-N1

Polyclonal antibody raised in rabbit against human ELAVL2 (ELAV Like RNA Binding Protein 2), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.

Lot001
Concentration1 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C.
Storage BufferPBS containing 50% glycerol, does not contain a preservative.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP 15 µg per 107 cells Fig 1
Western blotting 1:1,000 Fig 2
IP 5 µg per 2.5x105 cells Fig 3
  • Validation data

    ChIP

    RIP

    Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL2
    Immunoprecipitation was performed on total RNA isolated from 10 million 293T cells transfected with an ELAVL2 expression vector using 15 µg of the Diagenode antibody against ELAVL2 (Cat. No. C15410347) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL2 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    WB

    Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2
    Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (Cat. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2
    Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 µg of the Diagenode antibody against ELAVL2 (Cat. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.

  •  Applications
    RIP
    RIP Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  Documents
    ELAVL2 polyclonal antibody datasheet
    Polyclonal antibody raised in rabbit against human ELAVL2 (ELAV Like RNA Binding Protein 2), usin...
    Download
  •  Publications

    How to properly cite this product in your work

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